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WB (Western Blot)
(Figure 1. Western blot analysis of PICALM using anti-PICALM antibody.)
Rabbit PICALM Polyclonal Antibody | anti-PICALM antibody
Anti-PICALM Antibody
Gene Names
PICALM; LAP; CALM; CLTH
Reactivity
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
PICALM, Antibody; Anti-PICALM Antibody; Phosphatidylinositol-binding clathrin assembly protein; Clathrin assembly lymphoid myeloid leukemia protein; PICALM; CALM; anti-PICALM antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
652
Applicable Applications for anti-PICALM antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human PICALM recombinant protein (Position: M1-M652).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of PICALM using anti-PICALM antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of PICALM using anti-PICALM antibody.)
IHC (Immunohistochemisry)
IHC (Immunohistochemisry)
IF (Immunofluorescence)
IF (Immunofluorescence)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-PICALM antibody
Description: Rabbit IgG polyclonal antibody for PICALM detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.
Background: Phosphatidylinositol binding clathrin assembly protein, also known as PICALM, is a protein which in humans is encoded by the PICALM gene. It is mapped to 11q14.2. This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background: Phosphatidylinositol binding clathrin assembly protein, also known as PICALM, is a protein which in humans is encoded by the PICALM gene. It is mapped to 11q14.2. This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
References
1. Morris, S. A., Schroder, S., Plessmann, U., Weber, K., Ungewickell, E. Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site. EMBO J. 12: 667-675, 1993.
2. Stern, J. A., White, S. N., Lehmkuhl, L. B., Reina-Doreste, Y., Ferguson, J. L., Nascone-Yoder, N. M., Meurs, K. M. A single codon insertion in PICALM is associated with development of familial subvalvular aortic stenosis in Newfoundland dogs. Hum. Genet. 133: 1139-1148, 2014.
3. Treusch, S., Hamamichi, S., Goodman, J. L., Matlack, K. E. S., Chung, C. Y., Baru, V., Shulman, J. M., Parrado, A., Bevis, B. J., Valastyan, J. S., Han, H., Lindhagen-Persson, M., and 9 others. Functional links between amyloid-beta toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast. Science 334: 1241-1245, 2011.
2. Stern, J. A., White, S. N., Lehmkuhl, L. B., Reina-Doreste, Y., Ferguson, J. L., Nascone-Yoder, N. M., Meurs, K. M. A single codon insertion in PICALM is associated with development of familial subvalvular aortic stenosis in Newfoundland dogs. Hum. Genet. 133: 1139-1148, 2014.
3. Treusch, S., Hamamichi, S., Goodman, J. L., Matlack, K. E. S., Chung, C. Y., Baru, V., Shulman, J. M., Parrado, A., Bevis, B. J., Valastyan, J. S., Han, H., Lindhagen-Persson, M., and 9 others. Functional links between amyloid-beta toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast. Science 334: 1241-1245, 2011.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphatidylinositol-binding clathrin assembly protein isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol binding clathrin assembly protein
NCBI Official Symbol
PICALM
NCBI Official Synonym Symbols
LAP; CALM; CLTH
NCBI Protein Information
phosphatidylinositol-binding clathrin assembly protein
UniProt Protein Name
Phosphatidylinositol-binding clathrin assembly protein
UniProt Gene Name
PICALM
UniProt Synonym Gene Names
CALM
UniProt Entry Name
PICAL_HUMAN
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Product Notes
The PICALM picalm (Catalog #AAA125439) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PICALM Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PICALM can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PICALM picalm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PICALM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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