Rabbit Piccolo Polyclonal Antibody | anti-PCLO antibody

Piccolo Antibody: ATTO 594

Cat. Num. AAA103663

• Gene Names: PCLO; ACZ
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: Piccolo, Antibody; Piccolo Antibody: ATTO 594; ACZ; Aczonin; Brain-derived HLMN protein; DKFZp779G1236; KIAA0559; Multidomain presynaptic cytomatrix protein; PCLO; PCLO_HUMAN; Piccolo (presynaptic cytomatrix protein); Piccolo mouse homolog of Pico; Presynaptic cytomatrix protein; Protein piccolo; anti-PCLO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 4935

• Applicable Applications for anti-PCLO antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Conjugate: ATTO 594
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

WB (Western Blot)

(Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:1000.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.)

Related Product Information for anti-PCLO antibody

Background Info: Detects ~550kDa corresponding to the molecular weight of Piccolo. Multiple isoforms can be detected. No cross-reactivity to Bassoon.

Scientific Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Product Categories/Family for anti-PCLO antibody

Neuroscience

References

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.

NCBI and Uniprot Product Information

• NCBI GI #: 150170670
• NCBI GeneID: 27445
• NCBI Accession #: NP_055325.2
• NCBI GenBank Nucleotide #: NM_014510.2
• UniProt Accession #: Q9Y6VO; O43373; O60305; Q08E72; Q9BVC8; Q9UIV2; Q9Y6U9; A4D1A7; A6NNX9; Q9Y6V0
• Molecular Weight: 537,886 Da
• NCBI Official Full Name: protein piccolo isoform 2
• NCBI Official Synonym Full Names: piccolo presynaptic cytomatrix protein
• NCBI Official Symbol: PCLO
• NCBI Official Synonym Symbols: ACZ
• NCBI Protein Information: protein piccolo; aczonin; piccolo (presynaptic cytomatrix protein)
• UniProt Protein Name: Protein piccolo
• UniProt Gene Name: PCLO
• UniProt Synonym Gene Names: ACZ; KIAA0559
• UniProt Entry Name: PCLO_HUMAN