IHC (Immunohiostchemistry)
(PIGA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human PIGA Polyclonal Antibody | anti-PIGA antibody
PIGA Antibody (C-term)
Gene Names
PIGA; GPI3; PNH1; PIG-A; MCAHS2
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PIGA, Antibody; PIGA Antibody (C-term); Phosphatidylinositol N-acetylglucosaminyltransferase subunit A; GlcNAc-PI synthesis protein; Phosphatidylinositol-glycan biosynthesis class A protein; PIG-A; PIGA; anti-PIGA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PIGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-484 amino acids from the C-terminal region of human PIGA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
455-484
Sequence Length
250
Applicable Applications for anti-PIGA antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(PIGA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PIGA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PIGA Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIGA antibody detected the PIGA protein (arrow).)
WB (Western Blot)
(PIGA Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIGA antibody detected the PIGA protein (arrow).)
Related Product Information for anti-PIGA antibody
This gene encodes a protein required for synthesis of
N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first
intermediate in the biosynthetic pathway of GPI anchor. The GPI
anchor is a glycolipid found on many blood cells and which serves
to anchor proteins to the cell surface. Paroxysmal nocturnal
hemoglobinuria, an acquired hematologic disorder, has been shown to
result from mutations in this gene. Alternate splice variants have
been characterized. A related pseudogene is located on chromosome
12.
N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first
intermediate in the biosynthetic pathway of GPI anchor. The GPI
anchor is a glycolipid found on many blood cells and which serves
to anchor proteins to the cell surface. Paroxysmal nocturnal
hemoglobinuria, an acquired hematologic disorder, has been shown to
result from mutations in this gene. Alternate splice variants have
been characterized. A related pseudogene is located on chromosome
12.
Product Categories/Family for anti-PIGA antibody
References
Borowitz, M.J., et al. Cytometry B Clin Cytom 78(4):211-230(2010)
Peruzzi, B., et al. Mutat. Res. 705(1):3-10(2010)
Araten, D.J., et al. Mutat. Res. 686 (1-2), 1-8 (2010) :
Iida, Y., et al. Blood 83(11):3126-3131(1994)
Ware, R.E., et al. Blood 83(9):2418-2422(1994)
Peruzzi, B., et al. Mutat. Res. 705(1):3-10(2010)
Araten, D.J., et al. Mutat. Res. 686 (1-2), 1-8 (2010) :
Iida, Y., et al. Blood 83(11):3126-3131(1994)
Ware, R.E., et al. Blood 83(9):2418-2422(1994)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54127
NCBI Official Full Name
phosphatidylinositol N-acetylglucosaminyltransferase subunit A isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis, class A
NCBI Official Symbol
PIGA
NCBI Official Synonym Symbols
GPI3; PNH1; PIG-A; MCAHS2
NCBI Protein Information
phosphatidylinositol N-acetylglucosaminyltransferase subunit A
UniProt Protein Name
Phosphatidylinositol N-acetylglucosaminyltransferase subunit A
UniProt Gene Name
PIGA
UniProt Synonym Gene Names
PIG-A
UniProt Entry Name
PIGA_HUMAN
Similar Products
Product Notes
The PIGA piga (Catalog #AAA284984) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 455-484. The PIGA Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIGA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PIGA piga for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIGA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.