IP (Immunoprecipitation)
(Immunoprecipitating PIH1D1 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA229107 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA229107 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Rabbit anti-Human PIH1D1 Polyclonal Antibody | anti-PIH1D1 antibody
PIH1D1 Antibody
Gene Names
PIH1D1; NOP17
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity Purified
Synonyms
PIH1D1, Antibody; PIH1D1 Antibody; PIH1 domain-containing protein 1; PIH1D1; NOP17; Nucleolar protein 17 homolog; anti-PIH1D1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
290
Applicable Applications for anti-PIH1D1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human PIH1 domain-containing protein 1 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-168777 / sc-168778
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IP (Immunoprecipitation)
(Immunoprecipitating PIH1D1 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA229107 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA229107 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating PIH1D1 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA229107 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA229107 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells cells with AAA229107 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells cells with AAA229107 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta tissue using AAA229107 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta tissue using AAA229107 at dilution of 1:100)
Related Product Information for anti-PIH1D1 antibody
Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed:17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription (PubMed:22368283, PubMed:24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed:20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed:24036451).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,376 Da
NCBI Official Full Name
PIH1 domain-containing protein 1
NCBI Official Synonym Full Names
PIH1 domain containing 1
NCBI Official Symbol
PIH1D1
NCBI Official Synonym Symbols
NOP17
NCBI Protein Information
PIH1 domain-containing protein 1
UniProt Protein Name
PIH1 domain-containing protein 1
UniProt Gene Name
PIH1D1
UniProt Synonym Gene Names
NOP17
UniProt Entry Name
PIHD1_HUMAN
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Product Notes
The PIH1D1 pih1d1 (Catalog #AAA229107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIH1D1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIH1D1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PIH1D1 pih1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIH1D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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