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product-image-AAA40566_IF11.jpg IF (Immunofluorescence) (Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 μg/ml.)

Rabbit PIK3R4 Polyclonal Antibody | anti-PIK3R4 antibody

PIK3R4 Antibody

Gene Names
PIK3R4; p150; VPS15
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
PIK3R4 antibody is affinity chromatography purified via peptide column.
Synonyms
PIK3R4, Antibody; PIK3R4 Antibody; Phosphoinositide-3-kinase regulatory subunit 4; p150; Phosphoinositide 3-kinase adaptor protein; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; VPS15; phosphoinositide 3-kinase regulatory subunit 4; anti-PIK3R4 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PIK3R4 antibody is human, mouse and rat reactive.
Purity/Purification
PIK3R4 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1358
Applicable Applications for anti-PIK3R4 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4.
Buffer
PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PIK3R4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 μg/ml.)

product-image-AAA40566_IF11.jpg IF (Immunofluorescence) (Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 μg/ml.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 5 μg/ml.)

product-image-AAA40566_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 5 μg/ml.)

WB (Western Blot)

(Western blot analysis of PIK3R4 in human tonsil tissue lysate with PIK3R4 antibody at (A) 1 and (B) 2 μg/ml.)

product-image-AAA40566_WB15.gif WB (Western Blot) (Western blot analysis of PIK3R4 in human tonsil tissue lysate with PIK3R4 antibody at (A) 1 and (B) 2 μg/ml.)
Related Product Information for anti-PIK3R4 antibody
PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 149 kDa

Observed: 140 kDa
NCBI Official Full Name
phosphoinositide 3-kinase regulatory subunit 4
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 4
NCBI Official Symbol
PIK3R4
NCBI Official Synonym Symbols
p150; VPS15
NCBI Protein Information
phosphoinositide 3-kinase regulatory subunit 4; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; phosphoinositide 3-kinase adaptor protein; phosphatidylinositol 3-kinase-associated p150; phosphoinositide-3-kinase, regulatory subunit 4, p150
UniProt Protein Name
Phosphoinositide 3-kinase regulatory subunit 4
UniProt Gene Name
PIK3R4
UniProt Synonym Gene Names
PI3-kinase regulatory subunit 4
UniProt Entry Name
PI3R4_HUMAN

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Product Notes

The PIK3R4 pik3r4 (Catalog #AAA40566) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIK3R4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3R4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PIK3R4 pik3r4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3R4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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