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product-image-AAA289954_FCM11.jpg FCM/FACS (Flow Cytometry) (PIK4CB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit PIK4CB Polyclonal Antibody | anti-PI4KB antibody

PIK4CB Antibody (Center)

Gene Names
PI4KB; NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
Reactivity
Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PIK4CB, Antibody; PIK4CB Antibody (Center); Phosphatidylinositol 4-kinase beta; PI4K-beta; PI4Kbeta; PtdIns 4-kinase beta; NPIK; PI4K92; PI4KB; PIK4CB; anti-PI4KB antibody
Ordering
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PIK4CB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the Central region of human PIK4CB.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
519-548
Sequence Length
484
Applicable Applications for anti-PI4KB antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(PIK4CB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA289954_FCM11.jpg FCM/FACS (Flow Cytometry) (PIK4CB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

product-image-AAA289954_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of PIK4CB Antibody (Center) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.)

product-image-AAA289954_WB15.jpg WB (Western Blot) (Western blot analysis of PIK4CB Antibody (Center) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.)
Related Product Information for anti-PI4KB antibody
PIK4CB phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation.
Product Categories/Family for anti-PI4KB antibody
References
Jeganathan,S., et.al., Mol. Cell. Biol. 28 (14), 4549-4561 (2008) Pizarro-Cerda,J., et.al., Cell. Microbiol. 9 (10), 2381-2390 (2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
91379
NCBI Official Full Name
phosphatidylinositol 4-kinase beta isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase, catalytic, beta
NCBI Official Symbol
PI4KB
NCBI Official Synonym Symbols
NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
NCBI Protein Information
phosphatidylinositol 4-kinase beta
UniProt Protein Name
Phosphatidylinositol 4-kinase beta
UniProt Gene Name
PI4KB
UniProt Synonym Gene Names
PIK4CB; PI4K-beta; PI4Kbeta; PtdIns 4-kinase beta
UniProt Entry Name
PI4KB_HUMAN

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Product Notes

The PI4KB pi4kb (Catalog #AAA289954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 519-548. The PIK4CB Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PIK4CB can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PI4KB pi4kb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK4CB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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