Rabbit anti-Human PIM1 Polyclonal Antibody | anti-PIM1 antibody

PIM1 Antibody

Cat. Num. AAA233314

• Gene Names: PIM1; PIM
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, ELISA
• Purity: >95%, Protein G purified
• Synonyms: PIM1, Antibody; PIM1 Antibody; Serine/threonine-protein kinase pim-1; PIM1; anti-PIM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 404

• Applicable Applications for anti-PIM1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
• Immunogen: Recombinant Human Serine/threonine-protein kinase pim-1 protein (25-86AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IF (Immunofluorescence)

(Immunofluorescent analysis of Hela cells using AAA233314 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human prostate cancer using AAA233314 at dilution of 1:100)

Related Product Information for anti-PIM1 antibody

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B, induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.

NCBI and Uniprot Product Information

• NCBI GI #: 342307046
• NCBI GeneID: 5292
• NCBI Accession #: NP_001230115.1
• NCBI GenBank Nucleotide #: NM_001243186.1
• UniProt Accession #: P11309; Q38RT9; Q5T7H7; Q96RG3
• Molecular Weight: 35,686 Da
• NCBI Official Full Name: serine/threonine-protein kinase pim-1 isoform 1
• NCBI Official Synonym Full Names: Pim-1 proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: PIM1
• NCBI Official Synonym Symbols: PIM
• NCBI Protein Information: serine/threonine-protein kinase pim-1
• UniProt Protein Name: Serine/threonine-protein kinase pim-1
• UniProt Gene Name: PIM1