IHC (Immunohiostchemistry)
(AAA327705 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human PINX1 Polyclonal Antibody | anti-PINX1 antibody
PINX1 Antibody
Gene Names
PINX1; Gno1; LPTL; LPTS; Pxr1
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PINX1, Antibody; PINX1 Antibody; 67-11-3 protein; FLJ20565; Hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTL; LPTS; MGC8850; OTTHUMP00000224984; OTTHUMP00000224985; OTTHUMP00000224986; OTTHUMP00000225053; OTTHUMP00000225054; PIN2 interacting protein 1; Pin2-interacting protein X1; PIN2/TERF1-interacting telomerase inhibitor 1; PINX1; PINX1_HUMAN; Protein 67-11-3; TRF1-interacting protein 1; anti-PINX1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
PINX1 antibody detects endogenous levels of total PINX1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
174
Applicable Applications for anti-PINX1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PINX1
Subcellular Location
Nucleus. Nucleus > Nucleolus. Chromosome > Telomere. Chromosome > Centromere > Kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.
Tissue Specificity
Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
Predicted Cross Reactivity
Pig, Rabbit
Similarity
Pig (83%), Rabbit (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA327705 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327705 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of PINX1 using NIH-3T3 whole cell lysates)
WB (Western Blot)
(Western blot analysis of PINX1 using NIH-3T3 whole cell lysates)
Related Product Information for anti-PINX1 antibody
Function: Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.
Subunit Structure: Interacts with MCRS1, TERT, TERF1, NCL/nucleolin, and the telomerase RNA.
Similarity: The TID (telomerase inhibiting domain) domain is sufficient to bind TERT and inhibit its activity.The TBM domain mediates interaction with TERF1. Belongs to the PINX1 family.
Subunit Structure: Interacts with MCRS1, TERT, TERF1, NCL/nucleolin, and the telomerase RNA.
Similarity: The TID (telomerase inhibiting domain) domain is sufficient to bind TERT and inhibit its activity.The TBM domain mediates interaction with TERF1. Belongs to the PINX1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 37 kDa
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
PIN2/TERF1-interacting telomerase inhibitor 1 isoform 2
NCBI Official Synonym Full Names
PIN2 (TERF1) interacting telomerase inhibitor 1
NCBI Official Symbol
PINX1
NCBI Official Synonym Symbols
Gno1; LPTL; LPTS; Pxr1
NCBI Protein Information
PIN2/TERF1-interacting telomerase inhibitor 1
UniProt Protein Name
PIN2/TERF1-interacting telomerase inhibitor 1
UniProt Gene Name
PINX1
UniProt Synonym Gene Names
LPTL; LPTS
Similar Products
Product Notes
The PINX1 pinx1 (Catalog #AAA327705) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PINX1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PINX1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PINX1 pinx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PINX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.