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WB (Western Blot)
(Western blot analysis of lysates from COLO and Jurkat cells, using KAPC A/B Antibody. The lane on the right is blocked with the synthesized peptide.)
Rabbit PKA Polyclonal Antibody | anti-PKA antibody
PKA CAT Antibody
Gene Names
PRKACA; PKACA; PPNAD4
Reactivity
Human,Mouse,Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified
Synonyms
PKA, Antibody; PKA CAT Antibody; PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; anti-PKA antibody
Host
Rabbit
Reactivity
Human,Mouse,Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PKA CAT Antibody detects endogenous levels of total PKA CAT
Purity/Purification
Immunogen affinity purified
Form/Format
Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Positions
1-50
Applicable Applications for anti-PKA antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PKA CAT
Preparation and Storage
-20°C/1 year
WB (Western Blot)
(Western blot analysis of lysates from COLO and Jurkat cells, using KAPC A/B Antibody. The lane on the right is blocked with the synthesized peptide.)
WB (Western Blot)
(Western blot analysis of lysates from COLO and Jurkat cells, using KAPC A/B Antibody. The lane on the right is blocked with the synthesized peptide.)
WB (Western Blot)
(Western Blot analysis of various cells using PKAAlpha/Beta cat Polyclonal Antibody diluted at 1:1000.)
WB (Western Blot)
(Western Blot analysis of various cells using PKAAlpha/Beta cat Polyclonal Antibody diluted at 1:1000.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).)
WB (Western Blot)
(Western blot analysis of the lysates from RAW264.7cells using KAPC A/B antibody.)
WB (Western Blot)
(Western blot analysis of the lysates from RAW264.7cells using KAPC A/B antibody.)
Related Product Information for anti-PKA antibody
Background: Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36 kDa
NCBI Official Full Name
cAMP-dependent protein kinase catalytic subunit alpha isoform 3
NCBI Official Synonym Full Names
protein kinase cAMP-activated catalytic subunit alpha
NCBI Official Symbol
PRKACA
NCBI Official Synonym Symbols
PKACA; PPNAD4
NCBI Protein Information
cAMP-dependent protein kinase catalytic subunit alpha
UniProt Protein Name
cAMP-dependent protein kinase catalytic subunit alpha
UniProt Gene Name
PRKACA
UniProt Synonym Gene Names
PKACA; PKA C-alpha
Similar Products
Product Notes
The PKA prkaca (Catalog #AAA267895) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-50. The PKA CAT Antibody reacts with Human,Mouse,Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PKA can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PKA prkaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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