IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC beta2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit PKC beta2 Polyclonal Antibody | anti-PKC beta2 antibody
PKC beta2 Antibody (C-term)
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta
Reactivity
Human, mouse, rat, monkey
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PKC beta2, Antibody; PKC beta2 Antibody (C-term); PRKCB; PKCB; PRKCB1; Protein kinase C beta type; anti-PKC beta2 antibody
Host
Rabbit
Reactivity
Human, mouse, rat, monkey
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PKC beta2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 642-673 amino acids from the C-terminal region of human PKC beta2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 1.93 (varies by lot)
Sequence Positions
642-673
Sequence Length
673
Applicable Applications for anti-PKC beta2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC beta2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC beta2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(The anti-PKC beta2 Pab is used in Western blot to detect PKC beta2 in Jurkat cell lysate (lane 1) and mouse brain tissue lysate (lane 2).)
WB (Western Blot)
(The anti-PKC beta2 Pab is used in Western blot to detect PKC beta2 in Jurkat cell lysate (lane 1) and mouse brain tissue lysate (lane 2).)
Related Product Information for anti-PKC beta2 antibody
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC beta is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Isoform 1 uses an alternate exon at the 3' end compared to isoform 2, which includes a part of the coding region. The resulting isoform 1 has a distinct and shorter C-terminus, as compared to isoform 2.
References
Chen, D., et al., J. Biol. Chem. 279(6):4829-4839 (2004).
Hug, B.A., et al., J. Biol. Chem. 279(2):825-830 (2004).
Wu, H., et al., Biochem. Biophys. Res. Commun. 311(4):948-953 (2003).
Becker, K.P., et al., J. Biol. Chem. 278(52):52747-52754 (2003).
Birikh, K.R., et al., Proc. Natl. Acad. Sci. U.S.A. 100(1):283-288 (2003).
Hug, B.A., et al., J. Biol. Chem. 279(2):825-830 (2004).
Wu, H., et al., Biochem. Biophys. Res. Commun. 311(4):948-953 (2003).
Becker, K.P., et al., J. Biol. Chem. 278(52):52747-52754 (2003).
Birikh, K.R., et al., Proc. Natl. Acad. Sci. U.S.A. 100(1):283-288 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
77,012 Da
NCBI Official Full Name
protein kinase C beta type isoform 2
NCBI Official Synonym Full Names
protein kinase C, beta
NCBI Official Symbol
PRKCB
NCBI Official Synonym Symbols
PKCB; PRKCB1; PRKCB2; PKC-beta
NCBI Protein Information
protein kinase C beta type
UniProt Protein Name
Protein kinase C beta type
UniProt Gene Name
PRKCB
UniProt Synonym Gene Names
PKCB; PRKCB1; PKC-B; PKC-beta
UniProt Entry Name
KPCB_HUMAN
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Product Notes
The PKC beta2 prkcb (Catalog #AAA286631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 642-673. The PKC beta2 Antibody (C-term) reacts with Human, mouse, rat, monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PKC beta2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PKC beta2 prkcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC beta2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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