Rabbit PKCB1 Polyclonal Antibody | anti-PKCB1 antibody

Phospho-PKCB1 (Thr642+644) Antibody

Cat. Num. AAA328358

• Gene Names: PRKCB; PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
• Reactivity: Human, Mouse, Rat; Predicted: Bovine, Sheep, Rabbit
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: PKCB1, Antibody; Phospho-PKCB1 (Thr642+644) Antibody; KPCB_HUMAN; PKC beta; PKC-B; PKC-beta; PKCB; Prkcb; PRKCB I; PRKCB II; PRKCB1; PRKCB2; Protein kinase C beta 1; Protein kinase C beta 2; Protein kinase C beta; Protein kinase C beta type; protein kinase C, beta 1 polypeptide; anti-PKCB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Bovine, Sheep, Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-PKCB1(Thr642+644) Antibody detects endogenous levels of PKCB1 only when phosphorylated at Thr642+644.
• Purity/Purification: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 673

• Applicable Applications for anti-PKCB1 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human PKCB1 around the phosphorylation site of Thr642+644.
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of Phospho-PKCB1(Thr642+644) in lysates of HeLa heat shock, using Phospho-PKCB1(Thr642+644) Antibody .)

IHC (Immunohistochemisry)

(At 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohiostchemistry)

(At 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohistochemistry)

(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Related Product Information for anti-PKCB1 antibody

Description: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Post Translational Modifications: Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).

Subunit Structure: Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR.

Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 20127450
• NCBI GeneID: 5579
• NCBI Accession #: NP_002729.2
• NCBI GenBank Nucleotide #: NP_002729.2
• UniProt Accession #: P05771
• Molecular Weight: Observed: 77 kDa; Predicted: 77 kDa
• NCBI Official Full Name: protein kinase C beta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C beta
• NCBI Official Symbol: PRKCB
• NCBI Official Synonym Symbols: PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
• NCBI Protein Information: protein kinase C beta type
• UniProt Protein Name: Protein kinase C beta type
• UniProt Gene Name: PRKCB
• UniProt Synonym Gene Names: PKCB; PRKCB1; PKC-B; PKC-beta
• UniProt Entry Name: KPCB_HUMAN