IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody(, Red).)
Rabbit PKCbeta Polyclonal Antibody | anti-PKCbeta antibody
PKCbeta (Phospho-Thr641) Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Synonyms
PKCbeta, Antibody; PKCbeta (Phospho-Thr641) Antibody; PKCb (Phospho-Thr641) Antibody; PKCB; PRKCB1; PRKCB2; anti-PKCbeta antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of PKCb only when phosphorylated at threonine 641.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
673
Applicable Applications for anti-PKCbeta antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of threonine 641 (E-L-T(p)-P-T) derived from Human PKCb
Modification
Phospho-Thr641
Target Name
PKCb
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody(, Red).)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody(, Red).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCb(phospho-Thr641) antibody().)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCb(phospho-Thr641) antibody().)
WB (Western Blot)
(Western blot analysis of extracts from JK cells untreated or treated with PMA using PKCbeta (phospho-Thr641) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from JK cells untreated or treated with PMA using PKCbeta (phospho-Thr641) antibody.)
Related Product Information for anti-PKCbeta antibody
Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
Product Categories/Family for anti-PKCbeta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
77,012 Da
NCBI Official Full Name
protein kinase C beta type isoform 2
NCBI Official Synonym Full Names
protein kinase C, beta
NCBI Official Symbol
PRKCB
NCBI Official Synonym Symbols
PKCB; PRKCB1; PRKCB2; PKC-beta
NCBI Protein Information
protein kinase C beta type
UniProt Protein Name
Protein kinase C beta type
UniProt Gene Name
PRKCB
UniProt Synonym Gene Names
PKCB; PRKCB1; PKC-B; PKC-beta
UniProt Entry Name
KPCB_HUMAN
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Product Notes
The PKCbeta prkcb (Catalog #AAA304355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKCbeta (Phospho-Thr641) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PKCbeta can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PKCbeta prkcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKCbeta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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