WB (Western Blot)
(Detection of human PKM2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PKM2 antibody AAA212249 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. PKM2 was also immunoprecipitated by rabbit anti-PKM2 antibody A303-660A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
Rabbit anti-Human PKM2 Polyclonal Antibody | anti-PKM2 antibody
Rabbit anti-PKM2 Antibody, Affinity Purified
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PKM2, Antibody; Rabbit anti-PKM2 Antibody, Affinity Purified; pyruvate kinase 2/3; pyruvate kinase muscle isozyme; pyruvate kinase, muscle; TCB; THBP1; thyroid hormone-binding protein 1; tumor M2-PK; PKM2; pyruvate kinase isozymes M1/M2; thyroid hormone-binding protein, cytosolic; HEL-S-30; PK3; pyruvate kinase PKM; epididymis secretory protein Li 30; OIP3; OIP-3; OPA-interacting protein 3; p58; CTHBP; PK, muscle type; cytosolic thyroid hormone-binding protein; Pyruvate kinase PKM; pyruvate kinase M1/2; anti-PKM2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PKM2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PKM2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PKM2 antibody AAA212249 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. PKM2 was also immunoprecipitated by rabbit anti-PKM2 antibody A303-660A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
WB (Western Blot)
(Detection of human PKM2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PKM2 antibody AAA212249 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. PKM2 was also immunoprecipitated by rabbit anti-PKM2 antibody A303-660A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
IHC (Immunohiostchemistry)
(Detection of human PKM2 by immunohistochemistry. Samples: FFPE sections of human stomach. Antibody: Affinity purified rabbit anti-PKM2 Cat. No. AAA212249 Lot1 used at a dilution of 1:1,000 (1ug/ml) (left) and Cat. No. A303-660A Lot1 used at a dilution of 1:1,000 (1ug/ml) (right). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human PKM2 by immunohistochemistry. Samples: FFPE sections of human stomach. Antibody: Affinity purified rabbit anti-PKM2 Cat. No. AAA212249 Lot1 used at a dilution of 1:1,000 (1ug/ml) (left) and Cat. No. A303-660A Lot1 used at a dilution of 1:1,000 (1ug/ml) (right). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PKM2 by immunohistochemistry. Samples: FFPE sections of human osteosarcoma. Antibody: Affinity purified rabbit anti-PKM2 Cat. No. AAA212249 Lot1 used at a dilution of 1:1,000 (1ug/ml) (left) and Cat. No. A303-660A Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PKM2 by immunohistochemistry. Samples: FFPE sections of human osteosarcoma. Antibody: Affinity purified rabbit anti-PKM2 Cat. No. AAA212249 Lot1 used at a dilution of 1:1,000 (1ug/ml) (left) and Cat. No. A303-660A Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB)
Related Product Information for anti-PKM2 antibody
Pyruvate Kinase M2 (PKM2) is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a general role in caspase independent cell death of tumor cells [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14618].
Product Categories/Family for anti-PKM2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,273 Da
NCBI Official Full Name
pyruvate kinase PKM isoform a
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
NCBI Protein Information
pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; epididymis secretory protein Li 30; cytosolic
UniProt Protein Name
Pyruvate kinase PKM
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN
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Product Notes
The PKM2 pkm (Catalog #AAA212249) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PKM2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKM2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PKM2 pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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