FCM/FACS (Flow Cytometry)
(PLCB2 Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PLCB2 Polyclonal Antibody | anti-PLCB2 antibody
PLCB2 Antibody (N-term)
Gene Names
PLCB2; PLC-beta-2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PLCB2, Antibody; PLCB2 Antibody (N-term); 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2; Phosphoinositide phospholipase C-beta-2; Phospholipase C-beta-2; PLC-beta-2; PLCB2; anti-PLCB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PLCB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-202 amino acids from the N-terminal region of human PLCB2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
174-202
Sequence Length
1185
Applicable Applications for anti-PLCB2 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PLCB2 Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PLCB2 Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PLCB2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PLCB2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PLCB2 Antibody (N-term) in K562 cell line lysates (35ug/lane). PLCB2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PLCB2 Antibody (N-term) in K562 cell line lysates (35ug/lane). PLCB2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-PLCB2 antibody
PLCB2 is the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Product Categories/Family for anti-PLCB2 antibody
References
Grinberg,S., et.al., Am. J. Pathol. 175 (6), 2439-2453 (2009)
Xiao,W., et.al., Cancer Cell 16 (2), 161-171 (2009)
Xiao,W., et.al., Cancer Cell 16 (2), 161-171 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
134024
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform 1
NCBI Official Synonym Full Names
phospholipase C, beta 2
NCBI Official Symbol
PLCB2
NCBI Official Synonym Symbols
PLC-beta-2
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
UniProt Gene Name
PLCB2
UniProt Synonym Gene Names
PLC-beta-2
UniProt Entry Name
PLCB2_HUMAN
Similar Products
Product Notes
The PLCB2 plcb2 (Catalog #AAA288767) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 174-202. The PLCB2 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLCB2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PLCB2 plcb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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