WB (Western Blot)
(Figure 1. Western blot analysis of PLD2 using anti-PLD2 antibody.)
Rabbit PLD2 Polyclonal Antibody | anti-PLD2 antibody
Anti-PLD2 Antibody
Gene Names
PLD2; PLD1C
Reactivity
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
PLD2, Antibody; Anti-PLD2 Antibody; Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2; PLD2; anti-PLD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
922
Applicable Applications for anti-PLD2 antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E Coli-derived human PLD2 recombinant protein (Position: A37-E924).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of PLD2 using anti-PLD2 antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of PLD2 using anti-PLD2 antibody.)
IF (Immunofluorescence)
IF (Immunofluorescence)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-PLD2 antibody
Description: Rabbit IgG polyclonal antibody for PLD2 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.
Background: Phospholipase D2 is an enzyme that in humans is encoded by the PLD2 gene. It is mapped to 17p13.2. The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.
Background: Phospholipase D2 is an enzyme that in humans is encoded by the PLD2 gene. It is mapped to 17p13.2. The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.
References
1. Liscovitch, M., Czarny, M., Fiucci, G., Tang, X. Phospholipase D: molecular and cell biology of a novel gene family. Biochem. J. 345: 401-415, 2000.
2. Lopez, I., Arnold, R. S., Lambeth, J. D. Cloning and initial characterization of a human phospholipase D2 (hPLD2): ADP-ribosylation factor regulated hPLD2. J. Biol. Chem. 273: 12846-12852, 1998.
3. Park, S. H., Ryu, S. H., Suh, P. G., Kim, H. Assignment of human PLD2 to chromosome band 17p13.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 82: 225 only, 1998.
2. Lopez, I., Arnold, R. S., Lambeth, J. D. Cloning and initial characterization of a human phospholipase D2 (hPLD2): ADP-ribosylation factor regulated hPLD2. J. Biol. Chem. 273: 12846-12852, 1998.
3. Park, S. H., Ryu, S. H., Suh, P. G., Kim, H. Assignment of human PLD2 to chromosome band 17p13.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 82: 225 only, 1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phospholipase D2 isoform PLD2B
NCBI Official Synonym Full Names
phospholipase D2
NCBI Official Symbol
PLD2
NCBI Official Synonym Symbols
PLD1C
NCBI Protein Information
phospholipase D2
UniProt Protein Name
Phospholipase D2
UniProt Gene Name
PLD2
UniProt Synonym Gene Names
PLD 2; hPLD2
UniProt Entry Name
PLD2_HUMAN
Similar Products
Product Notes
The PLD2 pld2 (Catalog #AAA125451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PLD2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PLD2 pld2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.