WB (Western Blot)
(Western blot analysis of extracts from various samples, using PLDN antibody. Lane 1: HepG2 treated with blocking peptide. Lane 2: HepG2; Lane 3: 293;)
Rabbit PLDN Polyclonal Antibody | anti-PLDN antibody
PLDN Antibody
Gene Names
BLOC1S6; PA; HPS9; PLDN; BLOS6; PALLID
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PLDN, Antibody; PLDN Antibody; Bloc1s6; iogenesis of lysosome-related organelles complex 1 subunit 6; PA; Pallid (mouse) homolog; PALLID; Pallid protein; Pallid protein homolog; Pallidin; Pallidin homolog (mouse); PLDN; PLDN_HUMAN; Syntaxin 13 binding protein; Syntaxin 13-interacting protein; anti-PLDN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PLDN antibody detects endogenous levels of PLDN.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
172
Applicable Applications for anti-PLDN antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PLDN.
Subcellular Location
Cytoplasm. Endomembrane System. It can exist as a soluble protein as well as a peripheral membrane protein.
Tissue Specificity
Widely expressed.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (91%), Bovine (91%), Horse (91%), Sheep (91%), Rabbit (100%), Dog (82%), Chicken (82%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from various samples, using PLDN antibody. Lane 1: HepG2 treated with blocking peptide. Lane 2: HepG2; Lane 3: 293;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using PLDN antibody. Lane 1: HepG2 treated with blocking peptide. Lane 2: HepG2; Lane 3: 293;)
WB (Western Blot)
(Western blot analysis of extracts of K-562 cells, using PLDN antibody.)
WB (Western Blot)
(Western blot analysis of extracts of K-562 cells, using PLDN antibody.)
IHC (Immunohistochemistry)
(AAA322868 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322868 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PLDN antibody
Function: Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. May play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process.
Subunit Structure: Interacts with BLOC1S4 and DTNBP1/BLOC1S7 (By similarity). Homodimer. Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. Interacts with BLOC1S5, F-actin, SNAP25 isoform 1 and isoform 2, SNAP47 and STX12.
Post-translational Modifications: Phosphorylated.
Similarity: Belongs to the BLOC1S6 family.
Subunit Structure: Interacts with BLOC1S4 and DTNBP1/BLOC1S7 (By similarity). Homodimer. Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. Interacts with BLOC1S5, F-actin, SNAP25 isoform 1 and isoform 2, SNAP47 and STX12.
Post-translational Modifications: Phosphorylated.
Similarity: Belongs to the BLOC1S6 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 25 kDa
Predicted: 20 kDa
Predicted: 20 kDa
NCBI Official Full Name
biogenesis of lysosome-related organelles complex 1 subunit 6 isoform 2
NCBI Official Synonym Full Names
biogenesis of lysosomal organelles complex 1 subunit 6
NCBI Official Symbol
BLOC1S6
NCBI Official Synonym Symbols
PA; HPS9; PLDN; BLOS6; PALLID
NCBI Protein Information
biogenesis of lysosome-related organelles complex 1 subunit 6
UniProt Protein Name
Biogenesis of lysosome-related organelles complex 1 subunit 6
UniProt Gene Name
BLOC1S6
UniProt Synonym Gene Names
PA; PLDN; BLOC-1 subunit 6
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Product Notes
The PLDN bloc1s6 (Catalog #AAA322868) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLDN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLDN can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PLDN bloc1s6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLDN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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