IHC (Immunohistochemisry)
(PLEKHA4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLEKHA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human PLEKHA4 Polyclonal Antibody | anti-PLEKHA4 antibody
PLEKHA4 Antibody (N-term)
Gene Names
PLEKHA4; PEPP1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PLEKHA4, Antibody; PLEKHA4 Antibody (N-term); Pleckstrin homology domain-containing family A member 4; PH domain-containing family A member 4; Phosphoinositol 3-phosphate-binding protein 1; PEPP-1; PLEKHA4; PEPP1; anti-PLEKHA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PLEKHA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human PLEKHA4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (varies by lot)
Sequence Positions
21-50
Sequence Length
583
Applicable Applications for anti-PLEKHA4 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(PLEKHA4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLEKHA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(PLEKHA4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLEKHA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry)
(PLEKHA4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PLEKHA4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(PLEKHA4 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PLEKHA4 antibody detected the PLEKHA4 protein (arrow).)
WB (Western Blot)
(PLEKHA4 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PLEKHA4 antibody detected the PLEKHA4 protein (arrow).)
Related Product Information for anti-PLEKHA4 antibody
PLEKHA4 binds specifically to phosphatidylinositol-3-phosphate (PtdIns3P), but not to other phosphoinositides.
Product Categories/Family for anti-PLEKHA4 antibody
References
Stelzl, U., et al. Cell 122(6):957-968(2005)
Dowler, S., et al. Biochem. J. 351 (PT 1), 19-31 (2000) :
Dowler, S., et al. Biochem. J. 351 (PT 1), 19-31 (2000) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85401
NCBI Official Full Name
pleckstrin homology domain-containing family A member 4 isoform 2
NCBI Official Synonym Full Names
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
NCBI Official Symbol
PLEKHA4
NCBI Official Synonym Symbols
PEPP1
NCBI Protein Information
pleckstrin homology domain-containing family A member 4
UniProt Protein Name
Pleckstrin homology domain-containing family A member 4
UniProt Gene Name
PLEKHA4
UniProt Synonym Gene Names
PEPP1; PH domain-containing family A member 4; PEPP-1
UniProt Entry Name
PKHA4_HUMAN
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Product Notes
The PLEKHA4 plekha4 (Catalog #AAA290184) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-50. The PLEKHA4 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLEKHA4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PLEKHA4 plekha4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLEKHA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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