WB (Western Blot)
(Detection of human Plexin-A3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Plexin-A3 antibody AAA212716 (lot AAA212716-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human Plexin-A3 Polyclonal Antibody | anti-PLXNA3 antibody
Rabbit anti-Plexin-A3 Antibody, Affinity Purified
Gene Names
PLXNA3; 6.3; PLXN3; PLXN4; XAP-6; HSSEXGENE
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Plexin-A3, Antibody; Rabbit anti-Plexin-A3 Antibody, Affinity Purified; HSSEXGENE; PLXN4; XAP-6; Sex chromosome X transmembrane protein of HGF receptor family 3; semaphorin receptor SEX; plexin-4; plexin-A3; 6.3; PLXN3; Plexin-A3; plexin A3; anti-PLXNA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PLXNA3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1075 and 1125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Plexin-A3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Plexin-A3 antibody AAA212716 (lot AAA212716-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human Plexin-A3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Plexin-A3 antibody AAA212716 (lot AAA212716-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Plexin-A3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Plexin-A3 antibody AAA212716 (lot AAA212716-1) used for IP at 6 ug per reaction. Plexin-A3 was also immunoprecipitated by rabbit anti-Plexin-A3 antibody BL16810. For blotting immunoprecipitated Plexin-A3, AAA212716 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Plexin-A3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Plexin-A3 antibody AAA212716 (lot AAA212716-1) used for IP at 6 ug per reaction. Plexin-A3 was also immunoprecipitated by rabbit anti-Plexin-A3 antibody BL16810. For blotting immunoprecipitated Plexin-A3, AAA212716 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-PLXNA3 antibody
Plexin-A3 is a coreceptor for SEMA3A and SEMA3F. It is necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plexin-A3 plays a role in axon guidance in the developing nervous system. It regulates the migration of sympathetic neurons, but not of neural crest precursors. Plexin-A3 is required for normal dendrite spine morphology in pyramidal neurons. It may play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P51805].
Product Categories/Family for anti-PLXNA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
207,703 Da
NCBI Official Full Name
plexin-A3
NCBI Official Synonym Full Names
plexin A3
NCBI Official Symbol
PLXNA3
NCBI Official Synonym Symbols
6.3; PLXN3; PLXN4; XAP-6; HSSEXGENE
NCBI Protein Information
plexin-A3
UniProt Protein Name
Plexin-A3
UniProt Gene Name
PLXNA3
UniProt Synonym Gene Names
PLXN4; SEX
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Product Notes
The PLXNA3 plxna3 (Catalog #AAA212716) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Plexin-A3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Plexin-A3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PLXNA3 plxna3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Plexin-A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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