IHC (Immunohistochemistry)
(AAA31136 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
Rabbit PLK4 Polyclonal Antibody | anti-PLK4 antibody
PLK4 Antibody
Gene Names
PLK4; SAK; STK18; MCCRP2
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
PLK4, Antibody; PLK4 Antibody; PLK 4; PLK-4; PLK4; PLK4_HUMAN; Polo like Kinase 4; Polo-like kinase 4; Protein serine/threonine kinase; SAK; Serine/Threonine Kinase 18; Serine/threonine kinase; Serine/threonine protein kinase 18; Serine/threonine protein kinase PLK4; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase SAK; Snk Akin Kinase; STK 18; STK18; anti-PLK4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
938
Applicable Applications for anti-PLK4 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-3000
IHC: 1:50-1:200
*The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
*The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human PLK4, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA31136 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IHC (Immunohistochemistry)
(AAA31136 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
WB (Western Blot)
(Western blot analysis of PLK4 using COS7 whole cell lysates)
WB (Western Blot)
(Western blot analysis of PLK4 using COS7 whole cell lysates)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95 kDa
NCBI Official Full Name
serine/threonine-protein kinase PLK4 isoform 2
NCBI Official Synonym Full Names
polo like kinase 4
NCBI Official Symbol
PLK4
NCBI Official Synonym Symbols
SAK; STK18; MCCRP2
NCBI Protein Information
serine/threonine-protein kinase PLK4
UniProt Protein Name
Serine/threonine-protein kinase PLK4
UniProt Gene Name
PLK4
UniProt Synonym Gene Names
SAK; STK18; PLK-4
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Product Notes
The PLK4 plk4 (Catalog #AAA31136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLK4 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PLK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB: 1:1000-3000 IHC: 1:50-1:200 *The optimal dilution should be determined by the end user. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. . Researchers should empirically determine the suitability of the PLK4 plk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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