Rabbit PLN Polyclonal Antibody | anti-PLN antibody

Anti-PLN Antibody, Rabbit Polyclonal

Cat. Num. AAA256313

• Reactivity: Human, Cynomolgus
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: PLN, Antibody; Anti-PLN Antibody, Rabbit Polyclonal; PLN Antibody, Rabbit PAb, Antigen Affinity Purified; phospholamban; Anti-CMD1P Antibody; Anti-CMH18 Antibody; Anti-PLB Antibody; Anti-RP3-509L4.2 Antibody; anti-PLN antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PLN
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PLN antibody: IHC (Immunohistochemistry)
• Immunogen: E Coli-derived Human PLN fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PLN fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Immunochemical staining PLN in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining PLN in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PLN antibody

Phospholamban (PLN) is an important Ca2+ modulator at the sarcoplasmic reticulum (SR) of striated muscles. It physically interacts and inhibits sarcoplasmic reticulum Ca2+ ATPase (SERCA2) function, whereas a protein kinase A (PKA)-dependent phosphorylation at its serine 16 reverses the inhibition. Pathogenic variants in the human phospholamban coding gene (PLN) causes hereditary dilated cardiomyopathy with heart failure in an autosomal dominant mode. Pathogenic mutations in the phospholamban (PLN) gene may give rise to inherited cardiomyopathies due to its role in calcium homeostasis. Several PLN mutations have been identified, with the R14del mutation being the most prevalent cardiomyopathy-related mutation in the Netherlands. It is present in patients identifiesd with arrhythmogenic cardiomyopathy as well as dilated cardiomyopathy.

References

Truszkowska GT, et al. (2015) A study in polish patients with cardiomyopathy emphasizes pathogenicity of phospholamban (pln) mutations at amino acid position 9 and low penetrance of heterozygous null pln mutations. BMC Med Genet 16 21.Teng AC, et al. (2015) Metformin increases degradation of phospholamban via autophagy in cardiomyocytes. Proc Natl Acad Sci U S A 112 (23): 7165-7170.Hof IE, et al. (2019) Prevalence and cardiac phenotype of patients with a phospholamban mutation. Neth Heart J 27 (2): 64-69.Hakem Zadeh F, et al. (2019) Akap6 and phospholamban colocalize and interact in hek-293t cells and primary murine cardiomyocytes. Physiol Rep 7 (14): e14144.