Rabbit anti-Human PLOD3 Polyclonal Antibody | anti-PLOD3 antibody

PLOD3 Antibody (N-term)

Cat. Num. AAA285972

• Gene Names: PLOD3; LH3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: PLOD3, Antibody; PLOD3 Antibody (N-term); Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3; Lysyl hydroxylase 3; LH3; PLOD3; anti-PLOD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This PLOD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-105 amino acids from the N-terminal region of human PLOD3.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 78-105
• Sequence Length: 738

• Applicable Applications for anti-PLOD3 antibody: ELISA, WB (Western Blot)
• Function: Multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen (PubMed:11956192, PubMed:12475640. PubMed:18298658, PubMed:30089812, PubMed:18834968). Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:9582318, PubMed:9724729, PubMed:11956192, PubMed:12475640, PubMed:18298658, PubMed:30089812, PubMed:18834968). Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine (PubMed:12475640, PubMed:18298658, PubMed:30089812, PubMed:18834968). Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:10934207, PubMed:11896059, PubMed:11956192, PubMed:12475640, PubMed:18298658, PubMed:30089812, PubMed:18834968). Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen- like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5- hydroxylysine residues (PubMed:25419660). Essential for normal biosynthesis and secretion of type IV collagens (PubMed:18834968) (Probable). Essential for normal formation of basement membranes (By similarity).
• Cellular Location: Rough endoplasmic reticulum. Endoplasmic reticulum lumen. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9R0E1}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9R0E1}; Lumenal side {ECO:0000250|UniProtKB:Q9R0E1}. Secreted Secreted, extracellular space {ECO:0000250|UniProtKB:Q9R0E1}. Note=The majority of the secreted protein is associated with the extracellular matrix. {ECO:0000250|UniProtKB:Q9R0E1}
• Tissue Location: Ubiquitous (PubMed:9724729). Detected in heart, placenta and pancreas and at lower levels in lung, liver and skeletal muscle (PubMed:9582318, PubMed:9724729).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(PLOD3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PLOD3 antibody detected the PLOD3 protein (arrow).)

Related Product Information for anti-PLOD3 antibody

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity.

References

Wang, C., et al. J. Cell. Mol. Med. 13(3):508-521(2009)
Salo, A.M., et al. Am. J. Hum. Genet. 83(4):495-503(2008)
Salo, A.M., et al. J. Cell. Physiol. 207(3):644-653(2006)
Wang, C., et al. Matrix Biol. 21(7):559-566(2002)
Rautavuoma, K., et al. J. Biol. Chem. 277(25):23084-23091(2002)

NCBI and Uniprot Product Information

• NCBI GI #: 4505891
• NCBI GeneID: 8985
• NCBI Accession #: NP_001075.1
• NCBI GenBank Nucleotide #: NM_001084.4
• UniProt Accession #: O60568; Q540C3; B2R6W6
• Molecular Weight: 84785
• NCBI Official Full Name: procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
• NCBI Official Synonym Full Names: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• NCBI Official Symbol: PLOD3
• NCBI Official Synonym Symbols: LH3
• NCBI Protein Information: procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
• UniProt Protein Name: Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
• UniProt Gene Name: PLOD3
• UniProt Synonym Gene Names: LH3
• UniProt Entry Name: PLOD3_HUMAN