FCM/FACS (Flow Cytometry)
(PLTP Antibody (C-term) (AAA287862) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PLTP Polyclonal Antibody | anti-PLTP antibody
PLTP Antibody (C-term)
Gene Names
PLTP; BPIFE; HDLCQ9
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PLTP, Antibody; PLTP Antibody (C-term); Phospholipid transfer protein; Lipid transfer protein II; PLTP; anti-PLTP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PLTP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the C-terminal region of human PLTP.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Length
405
Applicable Applications for anti-PLTP antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Region
451-479
Antigen Source
HUMAN
Function
Mediates the transfer of phospholipids and free cholesterol from triglyceride-rich lipoproteins (low density lipoproteins or LDL and very low density lipoproteins or VLDL) into high-density lipoproteins (HDL) as well as the exchange of phospholipids between triglyceride-rich lipoproteins themselves (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine (PubMed: ). Plays an important role in HDL remodeling which involves modulating the size and composition of HDL (PubMed: ). Also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion (PubMed: ). Two distinct forms of PLTP exist in plasma: an active form that can transfer phosphatidylcholine from phospholipid vesicles to HDL, and an inactive form that lacks this capability (PubMed: ).
Tissue Location
Widely expressed. Highest level of expression in the ovary, thymus and placenta, with moderate levels found in the pancreas, small intestine, testis, lung and prostrate. Low level expression in the kidney, liver and spleen, with very low levels found in the heart, colon, skeletal muscle, leukocytes and brain. Expressed in the cortical neurons.
Cellular Location
Secreted. Nucleus.
Note: Nuclear export is XPO1/CRM1- dependent.
Note: Nuclear export is XPO1/CRM1- dependent.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PLTP Antibody (C-term) (AAA287862) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PLTP Antibody (C-term) (AAA287862) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PLTP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PLTP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PLTP antibody (C-term) (AAA287862) in HepG2 cell line lysates (35ug/lane). PLTP (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PLTP antibody (C-term) (AAA287862) in HepG2 cell line lysates (35ug/lane). PLTP (arrow) was detected using the purified Pab.)
Related Product Information for anti-PLTP antibody
PLTP is one of at least two lipid transfer proteins found in human plasma. The protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism.
Product Categories/Family for anti-PLTP antibody
References
Moerland,M., Samyn,H. Arterioscler. Thromb. Vasc. Biol. 28 (7), 1277-1282 (2008)
Albers,J.J., Wolfbauer,G. Biochim. Biophys. Acta 1258 (1), 27-34 (1995)
Albers,J.J., Wolfbauer,G. Biochim. Biophys. Acta 1258 (1), 27-34 (1995)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54739
NCBI Official Full Name
phospholipid transfer protein isoform c
NCBI Official Synonym Full Names
phospholipid transfer protein
NCBI Official Symbol
PLTP
NCBI Official Synonym Symbols
BPIFE; HDLCQ9
NCBI Protein Information
phospholipid transfer protein
UniProt Protein Name
Phospholipid transfer protein
UniProt Gene Name
PLTP
UniProt Entry Name
PLTP_HUMAN
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Product Notes
The PLTP pltp (Catalog #AAA287862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLTP Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLTP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PLTP pltp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLTP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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