IHC (Immunohiostchemistry)
(AAA322768 at 1/100 staining Human lung cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Rat PLUNC Polyclonal Antibody | anti-PLUNC antibody
PLUNC Antibody
Gene Names
BPIFA1; LUNX; NASG; PLUNC; SPURT; SPLUNC1; bA49G10.5
Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PLUNC, Antibody; PLUNC Antibody; BPIFA1; bA49G10.5; LPLUNC3; Lung-specific protein X; LUNX; NASG; Nasopharyngeal carcinoma-related protein; Palate lung and nasal epithelium clone protein; PLUNC; Protein Plunc; Secretory protein in upper respiratory tracts; SPLUNC1; SPURT; Tracheal epithelium-enriched protein; Von Ebner protein Hl; anti-PLUNC antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PLUNC antibody detects endogenous levels of total PLUNC
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
256
Applicable Applications for anti-PLUNC antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PLUNC
Subcellular Location
Extracellular Region or Secreted
Tissue Specificity
Lung, upper airways and nasopharyngeal regions, including trachea and nasal epithelium. Specifically expressed in the secretory ducts and submucosal glands of tracheobronchial tissues. Highest expression in the trachea and progressive decrease from proximal (bronchial) to distal (bronchiolar) airways. Also expressed in lung cancers and some other types of cancer.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA322768 at 1/100 staining Human lung cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA322768 at 1/100 staining Human lung cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of PLUNC using 293 whole cell lysates)
WB (Western Blot)
(Western blot analysis of PLUNC using 293 whole cell lysates)
Related Product Information for anti-PLUNC antibody
Function: Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus. Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression.
Subunit Structure: Monomer. Interacts (via N-terminus) with SCNN1B, a subunit of the heterotrimeric epithelial sodium channel (ENaC); this inhibits proteolytic activation of ENaC.
Post-translational Modifications: May be N-glycosylated.
Similarity: Belongs to the BPI/LBP/Plunc superfamily. Plunc family.
Subunit Structure: Monomer. Interacts (via N-terminus) with SCNN1B, a subunit of the heterotrimeric epithelial sodium channel (ENaC); this inhibits proteolytic activation of ENaC.
Post-translational Modifications: May be N-glycosylated.
Similarity: Belongs to the BPI/LBP/Plunc superfamily. Plunc family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 27 kDa
Predicted: 27 kDa
Predicted: 27 kDa
NCBI Official Full Name
BPI fold-containing family A member 1
NCBI Official Synonym Full Names
BPI fold containing family A member 1
NCBI Official Symbol
BPIFA1
NCBI Official Synonym Symbols
LUNX; NASG; PLUNC; SPURT; SPLUNC1; bA49G10.5
NCBI Protein Information
BPI fold-containing family A member 1
UniProt Protein Name
BPI fold-containing family A member 1
UniProt Gene Name
BPIFA1
UniProt Synonym Gene Names
LUNX; NASG; PLUNC; SPLUNC1; SPURT; SPLUNC1
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Product Notes
The PLUNC bpifa1 (Catalog #AAA322768) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLUNC Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLUNC can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PLUNC bpifa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLUNC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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