Rabbit PMVK Polyclonal Antibody | anti-PMVK antibody

PMVK Polyclonal Antibody

Cat. Num. AAA292006

• Gene Names: PMVK; PMK; PMKA; PMKASE; POROK1; HUMPMKI
• Reactivity: Rat, Pig, Bovine
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: affinity purified by Protein A
• Synonyms: PMVK, Antibody; PMVK Polyclonal Antibody; Phosphomevalonate kinase, PMKase, hPMK, 2.7.4.2, PMVK, PMKI; anti-PMVK antibody

• Host: Rabbit
• Reactivity: Rat, Pig, Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Heart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta and lung.
• Purity/Purification: affinity purified by Protein A
• Form/Format: Liquid; 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-PMVK antibody: ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: KLH conjugated synthetic peptide derived from human PMVK
• Epitope Specificity: 15-120/192
• Subcellular Location: Peroxisome
• Subunit: Monomer (By similarity).
• Function: Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid mediated biosynthesis of isopentenyl diphosphate and other polyisoprenoid metabolites.
• Cellular Location: Cytoplasm, cytosol
• Tissue Location: Heart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta and lung
• Preparation and Storage: Store at -20°Cfor one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C.

WB (Western Blot)

(Sample:Uterus (Mouse) Lysate at 40 ugCerebrum (Mouse) Lysate at 40 ugHeart (Mouse) Lysate at 40 ugLymph node (Mouse) Lysate at 40 ug293T(Human) Cell Lysate at 30 ugPrimary: Anti-PMVK (bs-5371R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 24 kD)

IHC (Immunohistochemistry)

(Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-PMVK Polyclonal Antibody, Unconjugated(bs-5371R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

Related Product Information for anti-PMVK antibody

PMVK is a 192 amino acid peroxisomal enzyme belonging to the nucleoside monophosphate (NMP) kinase family and is expressed in heart, liver, skeletal muscle, kidney, and pancreas with lower expression in brain, placenta and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway. PMVK exists as a monomer and is encoded by a gene located on human chromosome 1, which houses over 3,000 genes and is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 5729980
• NCBI GeneID: 10654
• NCBI Accession #: NP_006547.1
• NCBI GenBank Nucleotide #: NM_006556.3
• UniProt Accession #: Q15126; Q5TZW9
• Molecular Weight: 22 KDa
• NCBI Official Full Name: phosphomevalonate kinase
• NCBI Official Synonym Full Names: phosphomevalonate kinase
• NCBI Official Symbol: PMVK
• NCBI Official Synonym Symbols: PMK; PMKA; PMKASE; POROK1; HUMPMKI
• NCBI Protein Information: phosphomevalonate kinase
• UniProt Protein Name: Phosphomevalonate kinase
• UniProt Gene Name: PMVK
• UniProt Synonym Gene Names: PMKI; PMKase; hPMK
• UniProt Entry Name: PMVK_HUMAN