WB (Western Blot)
(Detection of human PNUTS by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, LNCaP, RKO, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody (AAA210950 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human PNUTS Polyclonal Antibody | anti-PNUTS antibody
Rabbit anti-PNUTS Antibody, Affinity Purified
Gene Names
PPP1R10; p99; FB19; R111; CAT53; PNUTS; PP1R10
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PNUTS, Antibody; Rabbit anti-PNUTS Antibody, Affinity Purified; protein phosphatase 1, regulatory (inhibitor) subunit 10; testis tissue sperm-binding protein Li 67n; R111; Protein FB19; PP1R10; PP1-binding protein of 114 kDa; phosphatase 1 nuclear targeting subunit; p99; MHC class I region proline-rich protein CAT53; HLA-C associated transcript 53; FB19; CAT53; PNUTS; serine/threonine-protein phosphatase 1 regulatory subunit 10; Serine/threonine-protein phosphatase 1 regulatory subunit 10; protein phosphatase 1 regulatory subunit 10; anti-PNUTS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PNUTS antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Monkey, Chimpanzee
Immunogen
Between 375 and 425
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PNUTS by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, LNCaP, RKO, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody (AAA210950 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PNUTS by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, LNCaP, RKO, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody (AAA210950 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PNUTS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PNUTS antibody (AAA210950 lot 4) used for IP at 6 ug per reaction. PNUTS was also immunoprecipitated by a previous lot of this antibody (AAA210950 lot 3) and a second antibody against a different epitope of PNUTS . For blotting immunoprecipitated PNUTS, AAA210950 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PNUTS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PNUTS antibody (AAA210950 lot 4) used for IP at 6 ug per reaction. PNUTS was also immunoprecipitated by a previous lot of this antibody (AAA210950 lot 3) and a second antibody against a different epitope of PNUTS . For blotting immunoprecipitated PNUTS, AAA210950 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-PNUTS antibody
PNUTS (PP1 nuclear targeting subunit) is a regulatory subunit of the PP1 protein phosphatase, a serine/threonine phosphatase involved in mitotic exit and chromosome decondensation. PNUTS has been shown to play a role in targeting PP1 to the nucleus and mediating PP1 association to specific RNAs.
Product Categories/Family for anti-PNUTS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99,058 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 1 regulatory subunit 10
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 10
NCBI Official Symbol
PPP1R10
NCBI Official Synonym Symbols
p99; FB19; R111; CAT53; PNUTS; PP1R10
NCBI Protein Information
serine/threonine-protein phosphatase 1 regulatory subunit 10; HLA-C associated transcript 53; PP1-binding protein of 114 kDa; phosphatase 1 nuclear targeting subunit; MHC class I region proline-rich protein CAT53; protein phosphatase 1, regulatory (inhibi
UniProt Protein Name
Serine/threonine-protein phosphatase 1 regulatory subunit 10
UniProt Gene Name
PPP1R10
UniProt Synonym Gene Names
CAT53; FB19; PNUTS
UniProt Entry Name
PP1RA_HUMAN
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Product Notes
The PNUTS ppp1r10 (Catalog #AAA210950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PNUTS Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNUTS can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PNUTS ppp1r10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNUTS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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