IHC (Immunohiostchemistry)
(AAA327954 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human POLA2 Polyclonal Antibody | anti-POLA2 antibody
POLA2 Antibody
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
POLA2, Antibody; POLA2 Antibody; AI573378; DNA directed DNA polymerase alpha 2; DNA polymerase alpha 70 kDa subunit; DNA polymerase alpha subunit B; DNA polymerase alpha subunit II; DNA polymerase subunit alpha B; DNA polymerase subunit II; DPOA2_HUMAN; FLJ21662; FLJ37250; polA2; Polymerase (DNA directed) alpha 2 (70kD subunit); Polymerase (DNA directed), alpha (70kD); Polymerase (DNA directed), alpha 2 accessory subunit; anti-POLA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
POLA2 antibody detects endogenous levels of total POLA2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
598
Applicable Applications for anti-POLA2 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human POLA2
Subcellular Location
Nucleus.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA327954 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327954 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of POLA2 using HuvEc whole cell lysates)
WB (Western Blot)
(Western blot analysis of POLA2 using HuvEc whole cell lysates)
Related Product Information for anti-POLA2 antibody
Function: May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery.
Subunit Structure: DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits. Subunit B binds to subunit A.
Post-translational Modifications: Phosphorylated in a cell cycle-dependent manner, in G2/M phase.
Similarity: The N-terminal 240 amino acids are sufficient to mediate complex formation. Belongs to the DNA polymerase alpha subunit B family.
Subunit Structure: DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits. Subunit B binds to subunit A.
Post-translational Modifications: Phosphorylated in a cell cycle-dependent manner, in G2/M phase.
Similarity: The N-terminal 240 amino acids are sufficient to mediate complex formation. Belongs to the DNA polymerase alpha subunit B family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 66 kDa
Predicted: 66 kDa
Predicted: 66 kDa
NCBI Official Full Name
DNA polymerase alpha subunit B
NCBI Official Synonym Full Names
DNA polymerase alpha 2, accessory subunit
NCBI Official Symbol
POLA2
NCBI Protein Information
DNA polymerase alpha subunit B
UniProt Protein Name
DNA polymerase alpha subunit B
UniProt Gene Name
POLA2
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Product Notes
The POLA2 pola2 (Catalog #AAA327954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLA2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLA2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the POLA2 pola2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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