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IHC (Immunohistochemistry)
(POLG Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit POLG Polyclonal Antibody | anti-POLG antibody
POLG Antibody (C-term)
Gene Names
POLG; PEO; MDP1; SCAE; MIRAS; POLG1; POLGA; SANDO; MTDPS4A; MTDPS4B
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
POLG, Antibody; POLG Antibody (C-term); DNA polymerase subunit gamma-1; Mitochondrial DNA polymerase catalytic subunit; PolG-alpha; POLG; MDP1; POLG1; POLGA; anti-POLG antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.46 (varies by lot)
Sequence Positions
1120-1148
Sequence Length
1,239
Applicable Applications for anti-POLG antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(POLG Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(POLG Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of POLG Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of POLG Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(POLG Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(POLG Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(POLG Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the POLG antibody detected the POLG protein (arrow).)
WB (Western Blot)
(POLG Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the POLG antibody detected the POLG protein (arrow).)
Related Product Information for anti-POLG antibody
Mitochondrial DNA polymerase is heterotrimeric, consisting
of a homodimer of accessory subunits plus a catalytic subunit. The
protein encoded by this gene is the catalytic subunit of
mitochondrial DNA polymerase. The encoded protein contains a
polyglutamine tract near its N-terminus that may be polymorphic.
Defects in this gene are a cause of progressive external
ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory
ataxic neuropathy dysarthria and ophthalmoparesis (SANDO),
Alpers-Huttenlocher syndrome (AHS), and mitochondrial
neurogastrointestinal encephalopathy syndrome (MNGIE). Two
transcript variants encoding the same protein have been found for
this gene.
of a homodimer of accessory subunits plus a catalytic subunit. The
protein encoded by this gene is the catalytic subunit of
mitochondrial DNA polymerase. The encoded protein contains a
polyglutamine tract near its N-terminus that may be polymorphic.
Defects in this gene are a cause of progressive external
ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory
ataxic neuropathy dysarthria and ophthalmoparesis (SANDO),
Alpers-Huttenlocher syndrome (AHS), and mitochondrial
neurogastrointestinal encephalopathy syndrome (MNGIE). Two
transcript variants encoding the same protein have been found for
this gene.
Product Categories/Family for anti-POLG antibody
References
Tong, Z.B., et al. Fertil. Steril. 94(7):2932-2934(2010)
Stewart, J.D., et al. Hepatology 52(5):1791-1796(2010)
Batabyal, D., et al. J. Biol. Chem. 285(44):34191-34201(2010)
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Stewart, J.D., et al. Hepatology 52(5):1791-1796(2010)
Batabyal, D., et al. J. Biol. Chem. 285(44):34191-34201(2010)
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
139562
NCBI Official Full Name
DNA polymerase subunit gamma-1
NCBI Official Synonym Full Names
polymerase (DNA directed), gamma
NCBI Official Symbol
POLG
NCBI Official Synonym Symbols
PEO; MDP1; SCAE; MIRAS; POLG1; POLGA; SANDO; MTDPS4A; MTDPS4B
NCBI Protein Information
DNA polymerase subunit gamma-1
UniProt Protein Name
DNA polymerase subunit gamma-1
UniProt Gene Name
POLG
UniProt Synonym Gene Names
MDP1; POLG1; POLGA
UniProt Entry Name
DPOG1_HUMAN
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Product Notes
The POLG polg (Catalog #AAA290183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1120-1148. The POLG Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's POLG can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the POLG polg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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