FCM/FACS (Flow Cytometry)
(POLG2 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human POLG2 Polyclonal Antibody | anti-POLG2 antibody
POLG2 Antibody (C-term)
Gene Names
POLG2; HP55; POLB; PEOA4; POLGB; MTPOLB; POLG-BETA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
POLG2, Antibody; POLG2 Antibody (C-term); DNA polymerase subunit gamma-2; mitochondrial; DNA polymerase gamma accessory 55 kDa subunit; p55; Mitochondrial DNA polymerase accessory subunit; MtPolB; PolG-beta; POLG2; MTPOLB; anti-POLG2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This POLG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-376 amino acids from the C-terminal region of human POLG2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
348-376
Sequence Length
485
Applicable Applications for anti-POLG2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(POLG2 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(POLG2 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with POLG2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with POLG2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of POLG2 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). POLG2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of POLG2 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). POLG2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-POLG2 antibody
The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit(POLG1) and a 55-kD accessory subunit (POLG2).
References
Longley,M.J., Am. J. Hum. Genet. 78 (6), 1026-1034 (2006)
Carrodeguas,J.A., J. Biol. Chem. 277 (51), 50008-50014 (2002)
Carrodeguas,J.A., J. Biol. Chem. 277 (51), 50008-50014 (2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54911
NCBI Official Full Name
DNA polymerase subunit gamma-2, mitochondrial
NCBI Official Synonym Full Names
polymerase (DNA directed), gamma 2, accessory subunit
NCBI Official Symbol
POLG2
NCBI Official Synonym Symbols
HP55; POLB; PEOA4; POLGB; MTPOLB; POLG-BETA
NCBI Protein Information
DNA polymerase subunit gamma-2, mitochondrial
UniProt Protein Name
DNA polymerase subunit gamma-2, mitochondrial
UniProt Gene Name
POLG2
UniProt Synonym Gene Names
MTPOLB; p55
UniProt Entry Name
DPOG2_HUMAN
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Product Notes
The POLG2 polg2 (Catalog #AAA289286) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 348-376. The POLG2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLG2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the POLG2 polg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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