WB (Western Blot)
(Detection of human POLR1A/RPA194 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-POLR1A/RPA194 antibody AAA212973 (lot AAA212973-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human POLR1A/RPA194 Polyclonal Antibody | anti-POLR1A/RPA194 antibody
Rabbit anti-POLR1A/RPA194 Antibody, Affinity Purified
Gene Names
POLR1A; A190; RPA1; RPO14; RPA194; RPO1-4
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
POLR1A/RPA194, Antibody; Rabbit anti-POLR1A/RPA194 Antibody, Affinity Purified; RNA polymerase I 194 kDa subunit; DNA-directed RNA polymerase I subunit A; RPO1-4; RPO14; RPA194; RPA1; RNA polymerase I subunit A1; polymerase (RNA) I polypeptide A, 194kDa; A190; DNA-directed RNA polymerase I subunit A1; polymerase (RNA) I subunit A; DNA-directed RNA polymerase I largest subunit; AFDCIN; DNA-directed RNA polymerase I subunit RPA1; RNA polymerase I subunit A; anti-POLR1A/RPA194 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-POLR1A/RPA194 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1425 and 1475
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human POLR1A/RPA194 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-POLR1A/RPA194 antibody AAA212973 (lot AAA212973-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human POLR1A/RPA194 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-POLR1A/RPA194 antibody AAA212973 (lot AAA212973-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human POLR1A/RPA194 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-POLR1A/RPA194 antibody AAA212973 (lot AAA212973-1) used for IP at 6 ug per reaction. POLR1A/RPA194 was also immunoprecipitated by rabbit anti-POLR1A/RPA194 antibody For blotting immunoprecipitated POLR1A/RPA194, AAA212973 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human POLR1A/RPA194 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-POLR1A/RPA194 antibody AAA212973 (lot AAA212973-1) used for IP at 6 ug per reaction. POLR1A/RPA194 was also immunoprecipitated by rabbit anti-POLR1A/RPA194 antibody For blotting immunoprecipitated POLR1A/RPA194, AAA212973 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-POLR1A/RPA194 antibody
POLR1A is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O95602].
Product Categories/Family for anti-POLR1A/RPA194 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
194,811 Da
NCBI Official Full Name
DNA-directed RNA polymerase I subunit RPA1
NCBI Official Synonym Full Names
polymerase (RNA) I polypeptide A, 194kDa
NCBI Official Symbol
POLR1A
NCBI Official Synonym Symbols
A190; RPA1; RPO14; RPA194; RPO1-4
NCBI Protein Information
DNA-directed RNA polymerase I subunit RPA1; RNA polymerase I subunit A1; RNA polymerase I 194 kDa subunit; DNA-directed RNA polymerase I subunit A; DNA-directed RNA polymerase I subunit A1; DNA-directed RNA polymerase I largest subunit
UniProt Protein Name
DNA-directed RNA polymerase I subunit RPA1
UniProt Gene Name
POLR1A
UniProt Synonym Gene Names
RNA polymerase I subunit A1; RPA194
UniProt Entry Name
RPA1_HUMAN
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Product Notes
The POLR1A/RPA194 polr1a (Catalog #AAA212973) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-POLR1A/RPA194 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLR1A/RPA194 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the POLR1A/RPA194 polr1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR1A/RPA194, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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