IF (Immunofluorescence)
(Immunofluorescence staining of POLR3H in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human POLR3H polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
Rabbit anti-Human POLR3H Polyclonal Antibody | anti-POLR3H antibody
Anti-POLR3H Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
POLR3H, Antibody; Anti-POLR3H Antibody, Rabbit Polyclonal; POLR3H Antibody, Rabbit PAb, Antigen Affinity Purified; polymerase (RNA) III (DNA directed) polypeptide H (22.9kD); Anti-RP3-347H13.9 Antibody; Anti-RPC22.9 Antibody; Anti-RPC8 Antibody; anti-POLR3H antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human POLR3H
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-POLR3H antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
E Coli-derived Human POLR3H fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human POLR3H fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of POLR3H in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human POLR3H polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of POLR3H in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human POLR3H polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human POLR3H in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human POLR3H in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human POLR3H in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human POLR3H in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-POLR3H antibody
POLR3H (RNA Polymerase III Subunit H) is a Protein Coding gene. The POLR3H gene, located on 22q13.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, A.thaliana, rice, and frog. POLR3H is a Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. POLR3H is widely expressed in the heart, kidney, and other tissues. Diseases associated with POLR3H include 46, Xx Sex Reversal 1, and Infantile Cerebellar-Retinal Degeneration. Among its related pathways are RNA Polymerase II Transcription Initiation And Promoter Clearance and Transcription of tRNA. 241 organisms have orthologs with the human gene POLR3H.
References
Jang KL, et al. (1992) The human immunodeficiency virus tat protein increases the transcription of human alu repeated sequences by increasing the activity of the cellular transcription factor tfiiic. J Acquir Immune Defic Syndr (1988) 5 (11): 1142-1147.Greco-Stewart VS, et al. (2009) The hepatitis delta virus rna genome interacts with the human rna polymerases i and iii. Virology 386 (1): 12-15.Franca MM, et al. (2019) Exome sequencing reveals the polr3h gene as a novel cause of primary ovarian insufficiency. J Clin Endocrinol Metab 104 (7): 2827-2841.
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Product Notes
The POLR3H (Catalog #AAA256794) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-POLR3H Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLR3H can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the POLR3H for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR3H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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