IHC (Immunohistochemisry)
(PON1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PON1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human PON1 Polyclonal Antibody | anti-PON1 antibody
PON1 Antibody (Center)
Gene Names
PON1; ESA; PON; MVCD5
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PON1, Antibody; PON1 Antibody (Center); Serum paraoxonase/arylesterase 1; PON 1; Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1; PON1; PON; anti-PON1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PON1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human PON1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
118-145
Sequence Length
355
Applicable Applications for anti-PON1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(PON1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PON1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(PON1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PON1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry)
(PON1 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PON1 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of lysate from human blood plasma tissue lysate, using PON1 Antibody (Center). AAA284422 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from human blood plasma tissue lysate, using PON1 Antibody (Center). AAA284422 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-PON1 antibody
The enzyme encoded by this gene is an arylesterase that
mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an
organophosphorus anticholinesterase compound that is produced in
vivo by oxidation of the insecticide parathion. Polymorphisms in
this gene are a risk factor in coronary artery disease. The gene is
found in a cluster of three related paraoxonase genes at 7q21.3.
mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an
organophosphorus anticholinesterase compound that is produced in
vivo by oxidation of the insecticide parathion. Polymorphisms in
this gene are a risk factor in coronary artery disease. The gene is
found in a cluster of three related paraoxonase genes at 7q21.3.
Product Categories/Family for anti-PON1 antibody
References
Ergun, M.A., et al. Biochem. Genet. (2010) In press :
Cagirci, G., et al. J. Heart Valve Dis. 19(4):453-458(2010)
Mendonca, M.I., et al. Rev Port Cardiol 29(4):571-580(2010)
Hashemi, M., et al. Genet. Mol. Res. 9(3):1735-1741(2010)
Martinez, C., et al. BMC Neurol 10, 71 (2010) :
Cagirci, G., et al. J. Heart Valve Dis. 19(4):453-458(2010)
Mendonca, M.I., et al. Rev Port Cardiol 29(4):571-580(2010)
Hashemi, M., et al. Genet. Mol. Res. 9(3):1735-1741(2010)
Martinez, C., et al. BMC Neurol 10, 71 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39731
NCBI Official Full Name
serum paraoxonase/arylesterase 1
NCBI Official Synonym Full Names
paraoxonase 1
NCBI Official Symbol
PON1
NCBI Official Synonym Symbols
ESA; PON; MVCD5
NCBI Protein Information
serum paraoxonase/arylesterase 1
UniProt Protein Name
Serum paraoxonase/arylesterase 1
UniProt Gene Name
PON1
UniProt Synonym Gene Names
PON; PON 1; A-esterase 1
UniProt Entry Name
PON1_HUMAN
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Product Notes
The PON1 pon1 (Catalog #AAA284422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 118-145. The PON1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PON1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PON1 pon1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PON1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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