Rabbit Potassium Chloride Cotransporter (KCC2) (Ser940) Polyclonal Antibody | anti-KCC2 antibody

Anti-Phospho-Ser940 Potassium Chloride Cotransporter (KCC2)

Cat. Num. AAA72823

• Gene Names: Slc12a5; Kcc2
• Reactivity: Bovine, Canine, Human, Non-Human Primate, Zebrafish
• Applications: Western Blot
• Purity: Antigen Affinity Purified from Pooled Serum; (Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.)
• Synonyms: Potassium Chloride Cotransporter (KCC2) (Ser940), Antibody; Anti-Phospho-Ser940 Potassium Chloride Cotransporter (KCC2); anti-KCC2 antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Human, Non-Human Primate, Zebrafish
• Clonality: Polyclonal
• Specificity: Specific for the ~135k KCC2 protein phosphorylated at Ser940. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.
• Purity/Purification: Antigen Affinity Purified from Pooled Serum; (Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 1116

• Applicable Applications for anti-KCC2 antibody: WB (Western Blot)
• Species Tested: Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH).
• Biological Significance: KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it's rate of internalization from the plasma membrane (Lee et al., 2007).
• Preparation and Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without without freeze/thawing due to the presence of 50% glycerol. Stock solutions for a minimum of 1 year at -20°C.

Application Data

Related Product Information for anti-KCC2 antibody

Affinity purified rabbit polyclonal antibody

References

• Lee HH, Walker JA, Williams JR, Goodier RJ, Payne JA, Moss SJ (2007) Direct protein kinase C-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter KCC2. J Biol Chem. 2007 Oct 12;282(41):29777-84
• Lee HH, Deeb TZ, Walker JA, Davies PA, Moss SJ (2011) NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci. Jun;14(6):736-43

NCBI and Uniprot Product Information

• NCBI GI #: 19705463
• NCBI GeneID: 171373
• NCBI Accession #: NP_599190.1
• NCBI GenBank Nucleotide #: NM_134363.1
• UniProt Accession #: Q63633; A7Y821
• Molecular Weight: 135kDa
• NCBI Official Full Name: solute carrier family 12 member 5
• NCBI Official Synonym Full Names: solute carrier family 12 (potassium-chloride transporter), member 5
• NCBI Official Symbol: Slc12a5
• NCBI Official Synonym Symbols: Kcc2
• NCBI Protein Information: solute carrier family 12 member 5; rKCC2; K-Cl cotransporter 2; neuronal K-Cl cotransporter; solute carrier family 12, member 5; neuronal-specific K-Cl cotransporter; furosemide-sensitive K-Cl cotransporter; electroneutral potassium-chloride cotransporter 2
• UniProt Protein Name: Solute carrier family 12 member 5
• UniProt Gene Name: Slc12a5
• UniProt Synonym Gene Names: Kcc2; rKCC2
• UniProt Entry Name: S12A5_RAT