IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit PP2A alpha Polyclonal Antibody | anti-PPP2CA antibody
PP2A alpha Antibody (C-term)
Gene Names
PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
Reactivity
Human; Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Drosophila
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
PP2A alpha, Antibody; PP2A alpha Antibody (C-term); Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C; PPP2CA; anti-PPP2CA antibody
Host
Rabbit
Reactivity
Human; Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Drosophila
Clonality
Polyclonal
Isotype
Ig
Specificity
This PP2A alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the C-terminal region of human PP2A alpha.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Length
309
Applicable Applications for anti-PPP2CA antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity).
Antigen Region
274-304 aa
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(All lanes : Anti-PPP2CA/B Antibody (F289) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PPP2CA/B Antibody (F289) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PPP2CA antibody
Description: PPP2CA/B represents the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.
Background: PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Background: PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Product Categories/Family for anti-PPP2CA antibody
References
Gergs, U., et al., J. Biol. Chem. 279 (39):40827-40834 (2004). Prickett, T.D., et al., J. Biol. Chem. 279 (37):38912-38920 (2004). Scott, G.K., et al., EMBO J. 22 (23):6234-6244 (2003). Rao, R.K., et al., Biochem. Biophys. Res. Commun. 293 (1):610-616 (2002). Avdi, N.J., et al., J. Biol. Chem. 277 (43):40687-40696 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35594 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
NCBI Official Synonym Full Names
protein phosphatase 2 catalytic subunit alpha
NCBI Official Symbol
PPP2CA
NCBI Official Synonym Symbols
RP-C; PP2Ac; PP2CA; PP2Calpha
NCBI Protein Information
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Gene Name
PPP2CA
UniProt Synonym Gene Names
PP2A-alpha; RP-C
UniProt Entry Name
PP2AA_HUMAN
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Product Notes
The PPP2CA ppp2ca (Catalog #AAA290720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP2A alpha Antibody (C-term) reacts with Human; Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's PP2A alpha can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PPP2CA ppp2ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2A alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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