WB (Western Blot)
(Detection of human and mouse PP2A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody AAA211115 (lot AAA211115-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse PP2A Polyclonal Antibody | anti-PP2A antibody
Rabbit anti-PP2A Antibody, Affinity Purified
Gene Names
PPP2CB; PP2CB; PP2Abeta
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PP2A, Antibody; Rabbit anti-PP2A Antibody, Affinity Purified; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; serine/threonine-protein phosphatase 2A catalytic subunit beta isoform; serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform; protein phosphatase type 2A catalytic subunit; testicular tissue protein Li 146; protein phosphatase 2A catalytic subunit, beta isoform; PP2CB; PP2A-beta; PP2Abeta; protein phosphatase 2, catalytic subunit, beta isozyme; protein phosphatase 2, catalytic subunit, beta isoform; Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform; protein phosphatase 2 catalytic subunit beta; anti-PP2A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PP2A antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, D. melanogaster, Chicken, Bovine, Rabbit, Pig
Immunogen
Between 259 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PP2A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody AAA211115 (lot AAA211115-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse PP2A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody AAA211115 (lot AAA211115-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody AAA211115 (lot AAA211115-2) used for IP at 6 ug per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (AAA211115-1) and rabbit anti-PP2A antibody For blotting immunoprecipitated PP2A, AAA211115 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody AAA211115 (lot AAA211115-2) used for IP at 6 ug per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (AAA211115-1) and rabbit anti-PP2A antibody For blotting immunoprecipitated PP2A, AAA211115 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-PP2A antibody
PP2A is a major serine/threonine phosphatase that is implicated in the negative control of cell cycle progression. PP2A encodes the beta isoform of the catalytic subunit (PP2CB). Chk2 has been shown to be negatively regulated and dephosphorylated by PP2A. Several proteins have been shown to associate with PP2A and regulate its activity such as eRF1, Hox11, CK2, and Igbp1/alpha4. Rapamycin has been shown to dissociate PP2A from alpha-4 and inhibit proliferation. This result suggests an alternative positive regulatory role of PP2A in cell proliferation.
Product Categories/Family for anti-PP2A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~59 kDa
NCBI Official Synonym Full Names
protein phosphatase 2, catalytic subunit, beta isozyme
NCBI Official Symbol
PPP2CB
NCBI Official Synonym Symbols
PP2CB; PP2Abeta
NCBI Protein Information
serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
UniProt Gene Name
PPP2CB
UniProt Synonym Gene Names
PP2A-beta
UniProt Entry Name
PP2AB_HUMAN
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Product Notes
The PP2A ppp2cb (Catalog #AAA211115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PP2A Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PP2A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PP2A ppp2cb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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