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product-image-AAA258840_IHC10.png IHC (Immunohistochemistry) (Immunochemical staining of mouse PPARG in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Rabbit PPAR gamma /NR1C3 Polyclonal Antibody | anti-PPARgamma/NR1C3 antibody

Anti-PPAR gamma /NR1C3 Antibody, Rabbit Polyclonal

Reactivity
Mouse, Rat
(Species predicted to react based on 100% sequence homology)
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PPAR gamma /NR1C3, Antibody; Anti-PPAR gamma /NR1C3 Antibody, Rabbit Polyclonal; peroxisome proliferator-activated receptor gamma; Anti-Nr1c3 Antibody; Anti-PPAR-gamma Antibody; Anti-PPAR-gamma2 Antibody; Anti-PPARgamma Antibody; Anti-PPARgamma2 Antibody; anti-PPARgamma/NR1C3 antibody
Ordering
Host
Rabbit
Reactivity
Mouse, Rat
(Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PPARgamma/NR1C3 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the Mouse PPAR gamma /NR1C3
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse PPAR gamma /NR1C3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemistry)

(Immunochemical staining of mouse PPARG in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

product-image-AAA258840_IHC10.png IHC (Immunohistochemistry) (Immunochemical staining of mouse PPARG in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemisry)

(Immunochemical staining of mouse PPARG in mouse prostate with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

product-image-AAA258840_IHC11.png IHC (Immunohistochemisry) (Immunochemical staining of mouse PPARG in mouse prostate with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of mouse PPARG in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

product-image-AAA258840_IHC13.png IHC (Immunohiostchemistry) (Immunochemical staining of mouse PPARG in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-PPARG rabbit polyclonal antibody at 1:500 dilutionLane A: Raw264.7 Whole Cell LysateLane B: NIH/3T3 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDa)

product-image-AAA258840_WB15.png WB (Western Blot) (Anti-PPARG rabbit polyclonal antibody at 1:500 dilutionLane A: Raw264.7 Whole Cell LysateLane B: NIH/3T3 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDa)
Related Product Information for anti-PPARgamma/NR1C3 antibody
Peroxisome proliferator-activated receptor gamma (PPARG), a nuclear hormone receptor, plays a critical role in the lipid and glucose homeostasis, adipocyte differentiation, as well as intracellular insulin-signaling events. The peroxisome proliferator-activated receptor gamma (PPARgamma) regulates osteoblast and osteoclast differentiation, and is the molecular target of thiazolidinediones (TZDs), insulin sensitizers that enhance glucose utilization and adipocyte differentiation. Peroxisome proliferator-activated receptor gamma (PPARG) is a transcription factor involved in atherosclerosis and related diseases. Peroxisome proliferator-activated receptor gamma (PPARG) plays an important role in the pathogenesis and maintenance of essential hypertension (EH). The functional single nucleotide polymorphisms in peroxisome proliferator-activated receptor gamma (PPARG) gene were predicted to be correlated with the susceptibility of colorectal cancer (CRC).
References
Hamblin M, et al. (2009) PPARs and the Cardiovascular System. Antioxid Redox Signal. 11 (6): 1415-52.
Khateeb J, et al. (2010) Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-gamma pathway. Atherosclerosis. 208 (1): 119-25.
Fajas L, et al. (1997) The organization, promoter analysis, and expression of the human PPARgamma gene. J Biol Chem. 272 (30): 18779-89.

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Product Notes

The PPARgamma/NR1C3 (Catalog #AAA258840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPAR gamma /NR1C3 Antibody, Rabbit Polyclonal reacts with Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's PPAR gamma /NR1C3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PPARgamma/NR1C3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPAR gamma /NR1C3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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