WB (Western Blot)
(PPHLN1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PPHLN1 antibody detected the PPHLN1 protein (arrow).)
Rabbit anti-Human PPHLN1 Polyclonal Antibody | anti-PPHLN1 antibody
PPHLN1 Antibody (N-term)
Gene Names
PPHLN1; HSPC206; HSPC232
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PPHLN1, Antibody; PPHLN1 Antibody (N-term); Periphilin-1; Gastric cancer antigen Ga50; PPHLN1; anti-PPHLN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PPHLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-66 amino acids from the N-terminal region of human PPHLN1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.29 (varies by lot)
Sequence Positions
45-66
Sequence Length
348
Applicable Applications for anti-PPHLN1 antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(PPHLN1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PPHLN1 antibody detected the PPHLN1 protein (arrow).)
WB (Western Blot)
(PPHLN1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PPHLN1 antibody detected the PPHLN1 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with PPHLN1 Antibody (N-term). AAA287564 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with PPHLN1 Antibody (N-term). AAA287564 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-PPHLN1 antibody
Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Product Categories/Family for anti-PPHLN1 antibody
References
Imami K., et al. Anal. Sci. 24:161-166(2008).
Line A., et al. Br. J. Cancer 86:1824-1830(2002).
Kazerounian S., et al. J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H., et al. Genome Res. 10:1546-1560(2000).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Line A., et al. Br. J. Cancer 86:1824-1830(2002).
Kazerounian S., et al. J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H., et al. Genome Res. 10:1546-1560(2000).
Ota T., et al. Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52737
NCBI Official Full Name
periphilin-1 isoform 6
NCBI Official Synonym Full Names
periphilin 1
NCBI Official Symbol
PPHLN1
NCBI Official Synonym Symbols
HSPC206; HSPC232
NCBI Protein Information
periphilin-1
UniProt Protein Name
Periphilin-1
UniProt Gene Name
PPHLN1
UniProt Entry Name
PPHLN_HUMAN
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Product Notes
The PPHLN1 pphln1 (Catalog #AAA287564) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 45-66. The PPHLN1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPHLN1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the PPHLN1 pphln1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPHLN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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