WB (Western Blot)
(Detection of human PPM1G by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PPM1G antibody AAA211196 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
Rabbit anti-Human, Mouse PPM1G Polyclonal Antibody | anti-PPM1G antibody
Rabbit anti-PPM1G Antibody, Affinity Purified
Gene Names
PPM1G; PP2CG; PPP2CG; PP2CGAMMA
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPM1G, Antibody; Rabbit anti-PPM1G Antibody, Affinity Purified; PPP2CG; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase 2C isoform gamma; protein phosphatase 2C gamma isoform; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase 1C; PP2C-gamma; PP2CGAMMA; PP2CG; protein phosphatase 1G; Protein phosphatase 1G; protein phosphatase, Mg2+/Mn2+ dependent 1G; anti-PPM1G antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PPM1G antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 400 and 450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PPM1G by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PPM1G antibody AAA211196 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
WB (Western Blot)
(Detection of human PPM1G by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PPM1G antibody AAA211196 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).)
IHC (Immunohistochemistry)
(Detection of human and mouse PPM1G by immunohistochemistry. Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-PPM1G (Cat. No. AAA211196 Lot1) used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse PPM1G by immunohistochemistry. Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-PPM1G (Cat. No. AAA211196 Lot1) used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-PPM1G antibody
Members of the protein phosphatase 2C (PP2C) family of isoenzymes are serine/threonine protein phosphatases that act as monomers, are magnesium or manganese dependent, and are structurally distinct from PP1, PP2A, and PP2B protein phosphatases. There are 14 mammalian PP2C genes that encode for members of the PP2C family of isoenzymes. Members of this family include PP2C alpha (PPM1A), PP2C beta (PPM1B), PP2C gamma (PPM1G), and PP2C delta (PPM1D). Because PPM1G has been shown to bind and dephosphorylate histones H2A and H2B, it appears to play a role in chromatin remodeling in response to DNA damage.
Product Categories/Family for anti-PPM1G antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,272 Da
NCBI Official Full Name
protein phosphatase 1G
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent, 1G
NCBI Official Symbol
PPM1G
NCBI Official Synonym Symbols
PP2CG; PPP2CG; PP2CGAMMA
NCBI Protein Information
protein phosphatase 1G; PP2C, gamma; PP2C-gamma; protein phosphatase 1C; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 2C gamma isoform; protein phosp
UniProt Protein Name
Protein phosphatase 1G
UniProt Gene Name
PPM1G
UniProt Synonym Gene Names
PPM1C; PP2C-gamma
UniProt Entry Name
PPM1G_HUMAN
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Product Notes
The PPM1G ppm1g (Catalog #AAA211196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPM1G Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1G can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPM1G ppm1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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