IF (Immunofluorescence)
(Figure 2. IF analysis of PPM1J using anti-PPM1J antibody (AAA127872).PPM1J was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PPM1J Antibody (AAA127872) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit PPM1J Polyclonal Antibody | anti-PPM1J antibody
Anti-PPM1J Antibody Picoband
Gene Names
PPM1J; PP2CZ; PPP2CZ; PP2Czeta
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
PPM1J, Antibody; Anti-PPM1J Antibody Picoband; Transmembrane protein 240; kelch repeat and BTB domain containing 2; Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489; anti-PPM1J antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-PPM1J antibody
ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E coli-derived human PPM1J recombinant protein (Position: K59-D475).
Subcellular Localization
Cul3-RING ubiquitin ligase complex
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(Figure 2. IF analysis of PPM1J using anti-PPM1J antibody (AAA127872).PPM1J was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PPM1J Antibody (AAA127872) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of PPM1J using anti-PPM1J antibody (AAA127872).PPM1J was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PPM1J Antibody (AAA127872) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot)
(Figure 1. Western blot analysis of PPM1J using anti-PPM1J antibody (AAA127872).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hacat whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1J antigen affinity purified polyclonal antibody (#AAA127872) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPM1J at approximately 55 kDa. The expected band size for PPM1J is at 55 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of PPM1J using anti-PPM1J antibody (AAA127872).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hacat whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1J antigen affinity purified polyclonal antibody (#AAA127872) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPM1J at approximately 55 kDa. The expected band size for PPM1J is at 55 kDa.)
Related Product Information for anti-PPM1J antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-PPM1J antibody
References
1. Gross, M. B. Personal Communication. Baltimore, Md. 9/30/2014.2. Kashiwaba, M., Katsura, K., Ohnishi, M., Sasaki, M., Tanaka, H., Nishimune, Y., Kobayashi, T., Tamura, S. A novel protein phosphatase 2C family member (PP2C-zeta) is able to associate with ubiquitin conjugating enzyme 9. FEBS Lett. 538: 197-202, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,464 Da
NCBI Official Full Name
protein phosphatase 1J
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent, 1J
NCBI Official Symbol
PPM1J
NCBI Official Synonym Symbols
PP2CZ; PPP2CZ; PP2Czeta
NCBI Protein Information
protein phosphatase 1J; PP2C-zeta; protein phosphatase 1J (PP2C domain containing); protein phosphatase 2C isoform zeta; protein phosphatase 2C zeta; protein phosphatase 2a, catalytic subunit, zeta isoform
UniProt Protein Name
Protein phosphatase 1J
UniProt Gene Name
PPM1J
UniProt Synonym Gene Names
PPP2CZ; PP2C-zeta
UniProt Entry Name
PPM1J_HUMAN
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Product Notes
The PPM1J ppm1j (Catalog #AAA127872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPM1J Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1J can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PPM1J ppm1j for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1J, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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