WB (Western Blot)
(Anti-iASPP Antibody - Western Blot. Western blot of affinity purified anti-iASPP antibody shows detection of a band at ~100 kD (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1500 in 5% BLOTTO/PBS overnight at 4C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Rabbit anti-Human PPP1R13L / RAI / IASPP Polyclonal Antibody | anti-PPP1R13L antibody
Rabbit Polyclonal to Human PPP1R13L / RAI / IASPP
Gene Names
PPP1R13L; RAI; RAI4; IASPP; NKIP1
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
PPP1R13L / RAI / IASPP, Antibody; Rabbit Polyclonal to Human PPP1R13L / RAI / IASPP; Anti-PPP1R13L / RAI / IASPP Antibody (aa780-797) IHC-plus; PPP1R13L; IASPP; Inhibitor of ASPP protein; NFkB-interacting protein 1; NKIP1; RAI4; RAI; Retinoic acid induced 4; NFkB interacting protein 1; PPP1R13BL; Protein iASPP; RelA-associated inhibitor; PPP1R13B-like protein; Human PPP1R13L; anti-PPP1R13L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Amino acids 780-797 of human iASPP (isoform 1) protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
828
Applicable Applications for anti-PPP1R13L antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human PPP1R13L / IASPP.
Immunogen Type
Synthetic peptide
Immunogen
PPP1R13L / RAI / IASPP antibody was raised against synthetic peptide from human PPP1R13L / IASPP.
Antigen Modification
aa780-797
Preparation and Storage
Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.
WB (Western Blot)
(Anti-iASPP Antibody - Western Blot. Western blot of affinity purified anti-iASPP antibody shows detection of a band at ~100 kD (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1500 in 5% BLOTTO/PBS overnight at 4C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
WB (Western Blot)
(Anti-iASPP Antibody - Western Blot. Western blot of affinity purified anti-iASPP antibody shows detection of a band at ~100 kD (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1500 in 5% BLOTTO/PBS overnight at 4C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
IHC (Immunohistochemistry)
(Anti-PPP1R13L / IASPP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-PPP1R13L / IASPP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,091 Da
NCBI Official Full Name
relA-associated inhibitor
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 13 like
NCBI Official Symbol
PPP1R13L
NCBI Official Synonym Symbols
RAI; RAI4; IASPP; NKIP1
NCBI Protein Information
relA-associated inhibitor; protein iASPP; PPP1R13B-like protein; retinoic acid induced 4; inhibitor of ASPP protein; NFkB interacting protein 1; NFkB-interacting protein 1; inhibitor of apoptosis stimulating protein of p53; protein phosphatase 1, regulatory (inhibitor) subunit 13 like
UniProt Protein Name
RelA-associated inhibitor
UniProt Gene Name
PPP1R13L
UniProt Synonym Gene Names
IASPP; NKIP1; PPP1R13BL; RAI; Protein iASPP
UniProt Entry Name
IASPP_HUMAN
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Product Notes
The PPP1R13L ppp1r13l (Catalog #AAA50738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human PPP1R13L / RAI / IASPP reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R13L / RAI / IASPP can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PPP1R13L ppp1r13l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1R13L / RAI / IASPP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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