WB (Western Blot)
(Detection of human PPP2R5C by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-PPP2R5C antibody AAA212293 (lot AAA212293-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PPP2R5C Polyclonal Antibody | anti-PPP2R5C antibody
Rabbit anti-PPP2R5C Antibody, Affinity Purified
Gene Names
PPP2R5C; B56G; PR61G; MGC23064; PPP2R5C
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPP2R5C, Antibody; Rabbit anti-PPP2R5C Antibody, Affinity Purified; PR61G; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform; renal carcinoma antigen NY-REN-29; protein phosphatase 2, regulatory subunit B', gamma isoform; protein phosphatase 2, regulatory subunit B (B56), gamma isoform; protein phosphatase 2 regulatory subunit B', gamma; PP2A B subunit isoform R5-gamma; PP2A B subunit isoform PR61-gamma; PP2A B subunit isoform B'-gamma; PP2A B subunit isoform B56-gamma; B56gamma; B' alpha regulatory subunit; B56G; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform; protein phosphatase 2 regulatory subunit B'gamma; anti-PPP2R5C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PPP2R5C antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 474 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PPP2R5C by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-PPP2R5C antibody AAA212293 (lot AAA212293-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PPP2R5C by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-PPP2R5C antibody AAA212293 (lot AAA212293-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PPP2R5C by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PPP2R5C antibody AAA212293 (lot AAA212293-1) used for IP at 6 ug/mg lysate. PPP2R5C was also immunoprecipitated by rabbit anti-PPP2R5C antibody which recognizes an upstream epitope. For blotting immunoprecipitated PPP2R5C, AAA212293 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP2R5C by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PPP2R5C antibody AAA212293 (lot AAA212293-1) used for IP at 6 ug/mg lysate. PPP2R5C was also immunoprecipitated by rabbit anti-PPP2R5C antibody which recognizes an upstream epitope. For blotting immunoprecipitated PPP2R5C, AAA212293 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human PPP2R5C by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PPP2R5C (Cat. No. AAA212293 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human PPP2R5C by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PPP2R5C (Cat. No. AAA212293 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-PPP2R5C antibody
Protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C) is a regulatory subunit of the PP2A phosphatase. The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13362].
Product Categories/Family for anti-PPP2R5C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform isoform a [Homo sapiens]
NCBI Official Synonym Full Names
protein phosphatase 2, regulatory subunit B', gamma
NCBI Official Symbol
PPP2R5C
NCBI Official Synonym Symbols
B56G; PR61G; MGC23064; PPP2R5C
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
UniProt Gene Name
PPP2R5C
UniProt Synonym Gene Names
KIAA0044
UniProt Entry Name
2A5G_HUMAN
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Product Notes
The PPP2R5C ppp2r5c (Catalog #AAA212293) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPP2R5C Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2R5C can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPP2R5C ppp2r5c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2R5C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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