WB (Western Blot)
(Western blot analysis of extracts from various samples, using PPP3CB Antibody.Lane 1: MCF7, treated with blocking peptide;Lane 2: MCF7;Lane 3: Mouse brain.)
Rabbit PPP3CB Polyclonal Antibody | anti-PPP3CB antibody
PPP3CB Antibody
Gene Names
PPP3CB; CNA2; CALNB; CALNA2; PP2Bbeta
Reactivity
Human, MousePredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PPP3CB, Antibody; PPP3CB Antibody; Calcineurin A beta; Calcineurin A2; Calcineurin B, formerly; Calmodulin dependent calcineurin A subunit beta isoform; Calmodulin-dependent calcineurin A subunit beta isoform; CALNA 2; CALNA2; CALNB; CAM PRP catalytic subunit; CAM-PRP catalytic subunit; CNA2; CnAbeta; PP2BB_HUMAN; PP2Bbeta; PPP3CB; Protein phosphatase 2B, catalytic subunit, beta isoform, formerly; Protein phosphatase 3 (formerly 2B) catalytic subunit beta isoform; Protein phosphatase 3 catalytic subunit beta isoform; Protein phosphatase 3 catalytic subunit beta isozyme; Serine/threonine protein phosphatase 2B catalytic subunit beta isoform; Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform; anti-PPP3CB antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
PPP3CB Antibody detects endogenous levels of PPP3CB.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
524
Applicable Applications for anti-PPP3CB antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human PPP3CB.
Fragment
Fab Fragment
Conjugate
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from various samples, using PPP3CB Antibody.Lane 1: MCF7, treated with blocking peptide;Lane 2: MCF7;Lane 3: Mouse brain.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using PPP3CB Antibody.Lane 1: MCF7, treated with blocking peptide;Lane 2: MCF7;Lane 3: Mouse brain.)
WB (Western Blot)
(Western blot analysis of extracts from HEK-293 cells, using PPP3CB Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HEK-293 cells, using PPP3CB Antibody. The lane on the left was treated with blocking peptide.)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Related Product Information for anti-PPP3CB antibody
Description: Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca2+-mediated signals (PubMed:19154138, PubMed:26794871). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138).
Subunit Structure: Forms a complex composed of a calmodulin-dependent catalytic subunit (also known as calcineurin A) and a regulatory Ca2+-binding subunit (also known as calcineurin B). There are three catalytic subunits, each encoded by a separate gene (PPP3CA, PPP3CB, and PPP3CC) and two regulatory subunits which are also encoded by separate genes (PPP3R1 and PPP3R2). In response to an increase in Ca2+ intracellular levels, forms a complex composed of PPP3CB/calcineurin A, calcineurin B and calmodulin. Interacts (via calcineurin B binding domain) with regulatory subunit PPP3R1/calcineurin B. Interacts (via calmodulin-binding domain) with calmodulin; the interaction depends on calmodulin binding to Ca2+.
Similarity: The poly-Pro domain may confer substrate specificity. The autoinhibitory domain prevents access to the catalytic site. The autoinhibitory segment prevents access to the substrate binding site. Belongs to the PPP phosphatase family. PP-2B subfamily.
Subunit Structure: Forms a complex composed of a calmodulin-dependent catalytic subunit (also known as calcineurin A) and a regulatory Ca2+-binding subunit (also known as calcineurin B). There are three catalytic subunits, each encoded by a separate gene (PPP3CA, PPP3CB, and PPP3CC) and two regulatory subunits which are also encoded by separate genes (PPP3R1 and PPP3R2). In response to an increase in Ca2+ intracellular levels, forms a complex composed of PPP3CB/calcineurin A, calcineurin B and calmodulin. Interacts (via calcineurin B binding domain) with regulatory subunit PPP3R1/calcineurin B. Interacts (via calmodulin-binding domain) with calmodulin; the interaction depends on calmodulin binding to Ca2+.
Similarity: The poly-Pro domain may confer substrate specificity. The autoinhibitory domain prevents access to the catalytic site. The autoinhibitory segment prevents access to the substrate binding site. Belongs to the PPP phosphatase family. PP-2B subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 59 kDa
Predicted: 60 kDa
Predicted: 60 kDa
NCBI Official Full Name
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
NCBI Official Synonym Full Names
protein phosphatase 3 catalytic subunit beta
NCBI Official Symbol
PPP3CB
NCBI Official Synonym Symbols
CNA2; CALNB; CALNA2; PP2Bbeta
NCBI Protein Information
serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
UniProt Gene Name
PPP3CB
UniProt Synonym Gene Names
CALNA2; CALNB; CNA2
UniProt Entry Name
PP2BB_HUMAN
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Product Notes
The PPP3CB ppp3cb (Catalog #AAA328704) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP3CB Antibody reacts with Human, Mouse Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's PPP3CB can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PPP3CB ppp3cb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP3CB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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