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WB (Western Blot)
(Detection of mouse PPP4R2 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4R2 antibody (AAA211172 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse PPP4R2 Polyclonal Antibody | anti-PPP4R2 antibody
Rabbit anti-PPP4R2 Antibody, Affinity Purified
Gene Names
PPP4R2; PP4R2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPP4R2, Antibody; Rabbit anti-PPP4R2 Antibody, Affinity Purified; PP4R2; serine/threonine-protein phosphatase 4 regulatory subunit 2; Serine/threonine-protein phosphatase 4 regulatory subunit 2; protein phosphatase 4 regulatory subunit 2; anti-PPP4R2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PPP4R2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 367 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse PPP4R2 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4R2 antibody (AAA211172 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse PPP4R2 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4R2 antibody (AAA211172 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PPP4R2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4R2 antibody (AAA211172 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PPP4R2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4R2 antibody (AAA211172 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP4R2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody (AAA211172 lot 3) used for IP at 6 ug per reaction. PPP4R2 was also immunoprecipitated by a previous lot of this antibody (AAA211172 lot 2) and a second antibody against a different epitope of PPP4R2 (BL3116). For blotting immunoprecipitated PPP4R2, AAA211172 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP4R2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody (AAA211172 lot 3) used for IP at 6 ug per reaction. PPP4R2 was also immunoprecipitated by a previous lot of this antibody (AAA211172 lot 2) and a second antibody against a different epitope of PPP4R2 (BL3116). For blotting immunoprecipitated PPP4R2, AAA211172 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human PPP4R2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PPP4R2 (AAA211172) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PPP4R2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PPP4R2 (AAA211172) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-PPP4R2 antibody
PPP4R2 is a regulatory subunit associated with the PP4 serine/threonine protein phosphatase catalytic subunit PPP4C. PPP4C is structurally related to PP2AC but does not associate with the same regulatory subunits. PPP4C associates with either the PPP4R1 or PPP4R2 regulatory subunit. PPP4R2 has been shown to localize to centrosomes and may function in targeting PPP4C to microtubule organizing centers.
Product Categories/Family for anti-PPP4R2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,898 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 4 regulatory subunit 2
NCBI Official Synonym Full Names
protein phosphatase 4, regulatory subunit 2
NCBI Official Symbol
PPP4R2
NCBI Official Synonym Symbols
PP4R2
NCBI Protein Information
serine/threonine-protein phosphatase 4 regulatory subunit 2
UniProt Protein Name
Serine/threonine-protein phosphatase 4 regulatory subunit 2
UniProt Gene Name
PPP4R2
UniProt Entry Name
PP4R2_HUMAN
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Product Notes
The PPP4R2 ppp4r2 (Catalog #AAA211172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPP4R2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPP4R2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPP4R2 ppp4r2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP4R2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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