IHC (Immunohistochemisry)
Rabbit PRAS40 Polyclonal Antibody | anti-PRAS40 antibody
PRAS40 Antibody
Gene Names
AKT1S1; Lobe; PRAS40
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA
Purity
Immobilized antigen affinity chromatography
Synonyms
PRAS40, Antibody; PRAS40 Antibody; Affinity Purified Proline-rich AKT1 substrate 1 isoform a Antibody; 40 kDa proline rich AKT substrate antibody; AKT1 S1 antibody; AKT1 substrate 1 (proline rich) antibody; AKT1S1 antibody; Lobe antibody; PRAS antibody; PRAS40 antibody; Proline rich Akt substrate 40 kDa antibody; Proline-rich AKT1 substrate 1 antibody; anti-PRAS40 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immobilized antigen affinity chromatography
Concentration
0.5 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
276
Applicable Applications for anti-PRAS40 antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA
Immunogen
Synthetic peptide taken within amino acid region 160-210 on human Proline-rich AKT1 substrate 1 isoform a protein.
Conjugation
Unconjugated
Molecular Function
negative regulation of cell size; negative regulation of protein kinase activity; negative regulation of TOR signaling; neurotrophin TRK receptor signaling pathway; regulation of cellular response to heat; regulation of neuron apoptotic process
Subcellular Location
Cytoplasm; Cytosol
Preparation and Storage
Store at -20 degree C for long term storage.
IHC (Immunohistochemisry)
IHC (Immunohistochemisry)
IHC (Immunohiostchemistry)
IHC (Immunohiostchemistry)
WB (Western Blot)
WB (Western Blot)
Related Product Information for anti-PRAS40 antibody
Overview: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Expression: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293)
Expression: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293)
Product Categories/Family for anti-PRAS40 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,262 Da
NCBI Official Full Name
proline-rich AKT1 substrate 1 isoform a
NCBI Official Synonym Full Names
AKT1 substrate 1
NCBI Official Symbol
AKT1S1
NCBI Official Synonym Symbols
Lobe; PRAS40
NCBI Protein Information
proline-rich AKT1 substrate 1
UniProt Protein Name
Proline-rich AKT1 substrate 1
UniProt Gene Name
AKT1S1
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Product Notes
The PRAS40 akt1s1 (Catalog #AAA226384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRAS40 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRAS40 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PRAS40 akt1s1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRAS40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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