Goat PRDX1 / Peroxiredoxin 1 Polyclonal Antibody | anti-PRDX1 antibody

Goat Polyclonal to Human PRDX1 / Peroxiredoxin 1

Cat. Num. AAA51453

• Gene Names: PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• Reactivity: Bat, Bovine, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PRDX1 / Peroxiredoxin 1, Antibody; Goat Polyclonal to Human PRDX1 / Peroxiredoxin 1; Anti-PRDX1 / Peroxiredoxin 1 Antibody (Internal) IHC-plus; PRDX1; MSP23; PAGB; Peroxiredoxin-1; PRX1; NKEFA; Peroxiredoxin 1; Thioredoxin peroxidase 2; NKEF-A; PAG; PRXI; TDPX2; Human PRDX1; anti-PRDX1 antibody

• Host: Goat
• Reactivity: Bat, Bovine, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
• Clonality: Polyclonal
• Specificity: Human PRDX1 / Peroxiredoxin 1. Reported variants represent identical protein (NP_002565.1, NP_859047.1, NP_859048.1).
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% sodium azide, 0.5% BSA,
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Positions: 106-117
• Sequence Length: 117

• Applicable Applications for anti-PRDX1 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Target: PRDX1 / Peroxiredoxin 1
• Epitope: aa106-117
• Immunogen: PRDX1 / Peroxiredoxin 1 antibody was raised against synthetic peptide C-SDPKRTIAQDYG from an internal region of human PRDX1 / Peroxiredoxin 1 (NP_859048.1).
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C . Avoid freeze-thaw cycles.

WB (Western Blot)

(Antibody (0.01 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohiostchemistry)

(Anti-PRDX1 / Peroxiredoxin 1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-PRDX1 / Peroxiredoxin 1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505591
• NCBI GeneID: 5052
• NCBI Accession #: NP_002565.1
• NCBI GenBank Nucleotide #: NM_002574.3
• UniProt Accession #: Q06830; P35703; Q2V576; Q5T154; Q5T155; B5BU26; D3DPZ8
• NCBI Official Full Name: peroxiredoxin-1
• NCBI Official Synonym Full Names: peroxiredoxin 1
• NCBI Official Symbol: PRDX1
• NCBI Official Synonym Symbols: PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• NCBI Protein Information: peroxiredoxin-1; thioredoxin peroxidase 2; proliferation-associated gene A; natural killer-enhancing factor A; proliferation-associated gene protein; natural killer cell-enhancing factor A; thioredoxin-dependent peroxide reductase 2
• UniProt Protein Name: Peroxiredoxin-1
• UniProt Gene Name: PRDX1
• UniProt Synonym Gene Names: PAGA; PAGB; TDPX2; NKEF-A; PAG
• UniProt Entry Name: PRDX1_HUMAN