Rabbit Pre-Pro-Vasopressin Polyclonal Antibody | anti-AVP antibody
Anti-Pre-Pro-Vasopressin
Reactivity
Human, Mouse, RatExpected Reactivity: Non-Human Primate
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Synonyms
Pre-Pro-Vasopressin, Antibody; Anti-Pre-Pro-Vasopressin; ADH antibody; Antidiuretic hormone antibody; Arginine vasopressin neurophysin II antibody; ARVP antibody; AVP antibody; AVP NPII antibody; copeptin antibody; Vasopressin neurophysin II copeptin antibody; VP antibody; Pre-Pro-Vasopressin; anti-AVP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Expected Reactivity: Non-Human Primate
Expected Reactivity: Non-Human Primate
Clonality
Polyclonal
Isotype
IgG
Specificity
Specific for locally produced levels of the ~ 20 kDa uncleaved pre-pro-vasopressin protein. Immunolabeling of the uncleaved pre-pro-vasopressin protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.
Form/Format
Liquid; Antigen Affinity Purified from Pooled Serum; 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-AVP antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Species
Human
Immunogen
Synthetic peptide corresponding to key amino acid residues surrounding the cleavage sites for the human vasopressin precursor peptide, conjugated to keyhole limpet hemocyanin (KLH).
Country of Origin
United States
Production Notes
Prepared from pooled rabbit serum by affinity purification on a column made with the peptide used as antigen.
Preparation and Storage
Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20 degree C.
After date of receipt, stable for at least 1 year at -20 degree C.
After date of receipt, stable for at least 1 year at -20 degree C.
Related Product Information for anti-AVP antibody
Pre-Pro-Vasopressin rabbit polyclonal primary antibody from the lab is produced in-house. It detects Pre-Pro-Vasopressin and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Vasopressin is a nine amino-acid peptide hormone that plays a key role in water and blood pressure homeostasis (Qureshi S., et al 2014). Vasopressin is the end-product of a highly processed 164 amino acid pre-pro-peptide. Processing of the vasopressin pre-pro-peptide results in three distinct peptides with a 1:1:1 ratio: vasopressin, neurophysin II, and copeptin (Arroyo J.P. et al, 2021). Vasopressin is the biologically active hormone, neurophysin II is a carrier molecule for vasopressin, and copeptin is the c-terminal glycosylated end-product. Vasopressin has been thought to be primarily made in the brain, and the sole source of vasopressin stimulating vasopressin V2 receptors in the kidney until recent studies (Arroyo JP, et al 2022). This antibody specially detects uncleaved pre-pro-vasopressin, accurately identifying locally produced vasopressin versus peripheral uptake of hypothalamic vasopressin or related peptides (Arroyo J.P. et al, 2022). This key research tool aided the Arroyo group in discovering immunoreactive vasopressin outside of the brain by recognizing localized vasopressin in human and mouse kidney, specifically in the distal nephron.
Vasopressin is a nine amino-acid peptide hormone that plays a key role in water and blood pressure homeostasis (Qureshi S., et al 2014). Vasopressin is the end-product of a highly processed 164 amino acid pre-pro-peptide. Processing of the vasopressin pre-pro-peptide results in three distinct peptides with a 1:1:1 ratio: vasopressin, neurophysin II, and copeptin (Arroyo J.P. et al, 2021). Vasopressin is the biologically active hormone, neurophysin II is a carrier molecule for vasopressin, and copeptin is the c-terminal glycosylated end-product. Vasopressin has been thought to be primarily made in the brain, and the sole source of vasopressin stimulating vasopressin V2 receptors in the kidney until recent studies (Arroyo JP, et al 2022). This antibody specially detects uncleaved pre-pro-vasopressin, accurately identifying locally produced vasopressin versus peripheral uptake of hypothalamic vasopressin or related peptides (Arroyo J.P. et al, 2022). This key research tool aided the Arroyo group in discovering immunoreactive vasopressin outside of the brain by recognizing localized vasopressin in human and mouse kidney, specifically in the distal nephron.
Product Categories/Family for anti-AVP antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The AVP avp (Catalog #AAA72763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Pre-Pro-Vasopressin reacts with Human, Mouse, Rat Expected Reactivity: Non-Human Primate and may cross-react with other species as described in the data sheet. AAA Biotech's Pre-Pro-Vasopressin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AVP avp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pre-Pro-Vasopressin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.