WB (Western Blot)
(Western blot analysis of PRKAG1 (arrow) using rabbit polyclonal PRKAG1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAG1 gene.)
Rabbit anti-Human, mouse PRKAG1 Polyclonal Antibody | anti-PRKAG1 antibody
PRKAG1 Antibody (N-term)
Gene Names
PRKAG1; AMPKG
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRKAG1, Antibody; PRKAG1 Antibody (N-term); 5'-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK subunit gamma-1; AMPKg; PRKAG1; anti-PRKAG1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PRKAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PRKAG1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
340
Applicable Applications for anti-PRKAG1 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of PRKAG1 (arrow) using rabbit polyclonal PRKAG1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAG1 gene.)
WB (Western Blot)
(Western blot analysis of PRKAG1 (arrow) using rabbit polyclonal PRKAG1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAG1 gene.)
WB (Western Blot)
(PRKAG1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).)
WB (Western Blot)
(PRKAG1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).)
WB (Western Blot)
(PRKAG1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).)
WB (Western Blot)
(PRKAG1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).)
Related Product Information for anti-PRKAG1 antibody
The protein encoded by this gene is a regulatory subunit
of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
consisting of an alpha catalytic subunit, and non-catalytic beta
and gamma subunits. AMPK is an important energy-sensing enzyme that
monitors cellular energy status. In response to cellular metabolic
stresses, AMPK is activated, and thus phosphorylates and
inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
regulating de novo biosynthesis of fatty acid and cholesterol. This
subunit is one of the gamma regulatory subunits of AMPK.
Alternatively spliced transcript variants encoding distinct
isoforms have been observed.
of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
consisting of an alpha catalytic subunit, and non-catalytic beta
and gamma subunits. AMPK is an important energy-sensing enzyme that
monitors cellular energy status. In response to cellular metabolic
stresses, AMPK is activated, and thus phosphorylates and
inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
regulating de novo biosynthesis of fatty acid and cholesterol. This
subunit is one of the gamma regulatory subunits of AMPK.
Alternatively spliced transcript variants encoding distinct
isoforms have been observed.
Product Categories/Family for anti-PRKAG1 antibody
References
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jassim, G., et al. Pharmacopsychiatry (2010) In press :
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jassim, G., et al. Pharmacopsychiatry (2010) In press :
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37579
NCBI Official Full Name
5'-AMP-activated protein kinase subunit gamma-1 isoform 3
NCBI Official Synonym Full Names
protein kinase, AMP-activated, gamma 1 non-catalytic subunit
NCBI Official Symbol
PRKAG1
NCBI Official Synonym Symbols
AMPKG
NCBI Protein Information
5'-AMP-activated protein kinase subunit gamma-1
UniProt Protein Name
5'-AMP-activated protein kinase subunit gamma-1
UniProt Gene Name
PRKAG1
UniProt Synonym Gene Names
AMPK gamma1; AMPK subunit gamma-1; AMPKg
UniProt Entry Name
AAKG1_HUMAN
Similar Products
Product Notes
The PRKAG1 prkag1 (Catalog #AAA289413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The PRKAG1 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRKAG1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PRKAG1 prkag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKAG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.