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product-image-AAA52389_WB10.jpg WB (Western Blot) (Western blot of PRKCA Antibody (N-term) in mouse NIH-3T3 cell line lysates (35 ug/lane). PRKCA (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse PRKCA / PKC-Alpha Polyclonal Antibody | anti-PRKCA antibody

Rabbit Polyclonal to Human PRKCA / PKC-Alpha

Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Ammonium sulfate precipitation
Synonyms
PRKCA / PKC-Alpha, Antibody; Rabbit Polyclonal to Human PRKCA / PKC-Alpha; Anti-PRKCA / PKC-Alpha Antibody (aa75-103) IHC-plus; PRKCA; Aging-associated gene 6; AAG6; Alpha PKC; PKC-A; Pkcalpha; Protein kinase C alpha type; PKC Alpha; PKC-alpha; PKCA; Protein kinase c alpha; Protein kinase C; alpha; Human PRKCA; PKC-Alpha; anti-PRKCA antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Human PKC Alpha
Purity/Purification
Ammonium sulfate precipitation
Form/Format
PBS, 0.09% sodium azide
Sequence Length
672
Applicable Applications for anti-PRKCA antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
KLH-conjugated synthetic peptide from N-terminal region of human PRKCA.
Immunogen Type
Synthetic peptide
Immunogen
PRKCA / PKC-Alpha antibody was raised against kLH-conjugated synthetic peptide from N-terminal region of human PRKCA.
Antigen Modification
aa75-103
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

WB (Western Blot)

(Western blot of PRKCA Antibody (N-term) in mouse NIH-3T3 cell line lysates (35 ug/lane). PRKCA (arrow) was detected using the purified Pab.)

product-image-AAA52389_WB10.jpg WB (Western Blot) (Western blot of PRKCA Antibody (N-term) in mouse NIH-3T3 cell line lysates (35 ug/lane). PRKCA (arrow) was detected using the purified Pab.)

FCM/FACS (Flow Cytometry)

(PRKCA Antibody (N-term) flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA52389_FCM11.jpg FCM/FACS (Flow Cytometry) (PRKCA Antibody (N-term) flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence)

(Confocal immunofluorescence of PRKCA Antibody (N-term)with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

product-image-AAA52389_IF13.jpg IF (Immunofluorescence) (Confocal immunofluorescence of PRKCA Antibody (N-term)with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry)

(Anti-PRKCA / PKC-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

product-image-AAA52389_IHC15.jpg IHC (Immunohistochemistry) (Anti-PRKCA / PKC-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Product Categories/Family for anti-PRKCA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
76,750 Da
NCBI Official Full Name
protein kinase C alpha type
NCBI Official Synonym Full Names
protein kinase C, alpha
NCBI Official Symbol
PRKCA
NCBI Official Synonym Symbols
AAG6; PKCA; PRKACA; PKC-alpha
NCBI Protein Information
protein kinase C alpha type; PKC-A; aging-associated gene 6
UniProt Protein Name
Protein kinase C alpha type
UniProt Gene Name
PRKCA
UniProt Synonym Gene Names
PKCA; PRKACA; PKC-A; PKC-alpha
UniProt Entry Name
KPCA_HUMAN

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Product Notes

The PRKCA prkca (Catalog #AAA52389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human PRKCA / PKC-Alpha reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRKCA / PKC-Alpha can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PRKCA prkca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKCA / PKC-Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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