WB (Western Blot)
(Detection of human PRKCQ by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-PRKCQ antibody AAA212560 (lot AAA212560-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PRKCQ Polyclonal Antibody | anti-PRKCQ antibody
Rabbit anti-PRKCQ Antibody, Affinity Purified
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PRKCQ, Antibody; Rabbit anti-PRKCQ Antibody, Affinity Purified; nPKC-theta; PRKCT; protein kinase C theta type; Protein kinase C theta type; protein kinase C theta; anti-PRKCQ antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PRKCQ antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 600 and 650
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PRKCQ by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-PRKCQ antibody AAA212560 (lot AAA212560-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PRKCQ by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-PRKCQ antibody AAA212560 (lot AAA212560-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PRKCQ by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-PRKCQ antibody AAA212560 (lot AAA212560-1) used for IP at 6 ug/mg lysate. PRKCQ was also immunoprecipitated by rabbit anti-PRKCQ antibody For blotting immunoprecipitated PRKCQ, AAA212560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PRKCQ by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-PRKCQ antibody AAA212560 (lot AAA212560-1) used for IP at 6 ug/mg lysate. PRKCQ was also immunoprecipitated by rabbit anti-PRKCQ antibody For blotting immunoprecipitated PRKCQ, AAA212560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-PRKCQ antibody
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. Protein kinase C theta type (PRKCQ) is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factor [taken from NCBI Entrez Gene (Gene ID: 5588)].
Product Categories/Family for anti-PRKCQ antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81,865 Da
NCBI Official Full Name
protein kinase C theta type isoform 1
NCBI Official Synonym Full Names
protein kinase C, theta
NCBI Official Symbol
PRKCQ
NCBI Official Synonym Symbols
PRKCT; nPKC-theta
NCBI Protein Information
protein kinase C theta type
UniProt Protein Name
Protein kinase C theta type
UniProt Gene Name
PRKCQ
UniProt Synonym Gene Names
PRKCT
UniProt Entry Name
KPCT_HUMAN
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Product Notes
The PRKCQ prkcq (Catalog #AAA212560) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PRKCQ Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKCQ can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PRKCQ prkcq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKCQ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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