WB (Western Blot)
(Detection of human and mouse PRKCSH by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRKCSH antibody AAA212904 (lot AAA212904-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse PRKCSH Polyclonal Antibody | anti-PRKCSH antibody
Rabbit anti-PRKCSH Antibody, Affinity Purified
Gene Names
PRKCSH; PCLD; PLD1; G19P1; PKCSH; AGE-R2
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PRKCSH, Antibody; Rabbit anti-PRKCSH Antibody, Affinity Purified; hepatocystin; VASAP-60; protein kinase C substrate, 80 Kda protein; protein kinase C substrate 60.1 kDa protein heavy chain; PLD1; PKCSH; PCLD; 80K-H protein; glucosidase 2 subunit beta; GIIB; G19P1; AGE-R2; AGE-binding receptor 2; advanced glycation end-product receptor 2; glucosidase II subunit beta; PCLD1; Glucosidase 2 subunit beta; protein kinase C substrate 80K-H; anti-PRKCSH antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PRKCSH antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PRKCSH by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRKCSH antibody AAA212904 (lot AAA212904-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse PRKCSH by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRKCSH antibody AAA212904 (lot AAA212904-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PRKCSH by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRKCSH antibody AAA212904 (lot AAA212904-1) used for IP at 6 ug per reaction. PRKCSH was also immunoprecipitated by rabbit anti-PRKCSH antibody BL18483. For blotting immunoprecipitated PRKCSH, AAA212904 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PRKCSH by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRKCSH antibody AAA212904 (lot AAA212904-1) used for IP at 6 ug per reaction. PRKCSH was also immunoprecipitated by rabbit anti-PRKCSH antibody BL18483. For blotting immunoprecipitated PRKCSH, AAA212904 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-PRKCSH antibody
Protein kinase C substrate 60.1 kDa protein heavy chain (PRKCSH) is a regulatory subunit of glucosidase II [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14314].
Product Categories/Family for anti-PRKCSH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,178 Da
NCBI Official Full Name
glucosidase 2 subunit beta isoform 1
NCBI Official Synonym Full Names
protein kinase C substrate 80K-H
NCBI Official Symbol
PRKCSH
NCBI Official Synonym Symbols
PCLD; PLD1; G19P1; PKCSH; AGE-R2
NCBI Protein Information
glucosidase 2 subunit beta; hepatocystin; AGE-binding receptor 2; glucosidase II subunit beta; protein kinase C substrate, 80 Kda protein; protein kinase C substrate 60.1 kDa protein heavy chain
UniProt Protein Name
Glucosidase 2 subunit beta
UniProt Gene Name
PRKCSH
UniProt Synonym Gene Names
G19P1; PKCSH
UniProt Entry Name
GLU2B_HUMAN
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Product Notes
The PRKCSH prkcsh (Catalog #AAA212904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PRKCSH Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRKCSH can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PRKCSH prkcsh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKCSH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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