WB (Western Blot)
(Detection of PRKRIR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP; 1/4 of IP reaction loaded) and mouse NIH 3T3 (M; 50 ug for WB) cells. Antibodies: Affinity purified rabbit anti-PRKRIR antibody AAA211035 (lot AAA211035-1) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). PRKRIR was also immunoprecipitated using rabbit anti-PRKRIR antibody BL2254 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
Rabbit anti-Human, Mouse PRKRIR Polyclonal Antibody | anti-PRKRIR antibody
Rabbit anti-PRKRIR Antibody, Affinity Purified
Gene Names
PRKRIR; DAP4; THAP0; THAP12; P52rIPK
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PRKRIR, Antibody; Rabbit anti-PRKRIR Antibody, Affinity Purified; inhibitor of protein kinase PKR; THAP0; THAP domain-containing protein 12; THAP domain-containing protein 0; testicular tissue protein Li 133; protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor); PRKRIR; P58IPK-regulatory protein; P52rIPK; death-associated protein 4; DAP4; 58 kDa interferon-induced protein kinase-interacting protein; 52 kDa repressor of the inhibitor of the protein kinase; p58IPK-interacting protein; THAP domain containing 12; anti-PRKRIR antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PRKRIR antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 175 and 225
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of PRKRIR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP; 1/4 of IP reaction loaded) and mouse NIH 3T3 (M; 50 ug for WB) cells. Antibodies: Affinity purified rabbit anti-PRKRIR antibody AAA211035 (lot AAA211035-1) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). PRKRIR was also immunoprecipitated using rabbit anti-PRKRIR antibody BL2254 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
WB (Western Blot)
(Detection of PRKRIR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP; 1/4 of IP reaction loaded) and mouse NIH 3T3 (M; 50 ug for WB) cells. Antibodies: Affinity purified rabbit anti-PRKRIR antibody AAA211035 (lot AAA211035-1) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). PRKRIR was also immunoprecipitated using rabbit anti-PRKRIR antibody BL2254 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human and mouse PRKRIR by immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-PRKRIR (Cat. No. AAA211035 Lot1) used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse PRKRIR by immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-PRKRIR (Cat. No. AAA211035 Lot1) used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-PRKRIR antibody
P58IPK is an inhibitor of the serine/threonine protein kinase, PKR. P58IPK functions to repress eukaryotic initiation factor 2-alpha subunit (eIF-2alpha) phosphorylation by PKR. PRKRIR, also designated P52RIPK, is a P58IPK interacting protein that abrogates P58IPK mediated suppression of PKR activity.
Product Categories/Family for anti-PRKRIR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,704 Da
NCBI Official Full Name
52 kDa repressor of the inhibitor of the protein kinase
NCBI Official Synonym Full Names
protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor)
NCBI Official Symbol
PRKRIR
NCBI Official Synonym Symbols
DAP4; THAP0; THAP12; P52rIPK
NCBI Protein Information
52 kDa repressor of the inhibitor of the protein kinase; THAP domain containing 12; death-associated protein 4; p58IPK-interacting protein; inhibitor of protein kinase PKR; THAP domain-containing protein 0; 58 kDa interferon-induced protein kinase-interac
UniProt Protein Name
52 kDa repressor of the inhibitor of the protein kinase
UniProt Gene Name
PRKRIR
UniProt Synonym Gene Names
DAP4; P52RIPK; THAP0; p52rIPK; p58IPK-interacting protein
UniProt Entry Name
P52K_HUMAN
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Product Notes
The PRKRIR prkrir (Catalog #AAA211035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PRKRIR Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRKRIR can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PRKRIR prkrir for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKRIR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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